Figure 3 : The catalytic activities of the AAA+ and eSTK domains of YeaG are required for its role in adaptation to sustained N starvation in E. coli.

From: Adaptation to sustained nitrogen starvation by Escherichia coli requires the eukaryote-like serine/threonine kinase YeaG

Figure 3

(A,B) Growth curves show complementation of the phenotype of the ∆yeaG mutant strain with plasmid-borne yeaG (pBAD18-yeaG), but not with mutant variants of yeaG containing catalytically deleterious mutations in the AAA+ domain (in A. pBAD18-yeaG K116A; pBAD18-yeaG R232A) or eSTK domain (in B. pBAD18-yeaG K426A; pBAD18-yeaG Y382Stop). Error bars on all growth curves represent sd (n = 3). Statistical analyses were performed by one-way ANOVA (**P < 0.01). (C) Protein levels of wild-type and mutant variants of YeaG assessed by anti-His immunoblotting.