Figure 2 : CD163 detection in in vitro models.

From: Targeted gold-coated iron oxide nanoparticles for CD163 detection in atherosclerosis by MRI

Figure 2

(A) Western blot of CD163 expression and α-actin (loading control) in THP-1 macrophages cell lysates with or without dexamethasone treatment for 24 hours. (B) Graph showing the normalized T2 values obtained from MRI images of human macrophages CD163 (+) and CD163 (−) incubated with NP-CD163(h) and NP-IgG(h), n  = 3. All the replicates were normalized to the blank (cells that have not been incubated with any nanoparticles). The error bars shows the SEM. $p < 0.05 versus Blank; *p < 0.05, **p < 0.01 and ***p < 0.001 versus NP-CD163(+) (C) Representative MRI phantoms of human macrophages CD163 (+) and CD163 (−) incubated with NP-CD163(h) and NP-IgG(h). (D) Immunodetection of CD163 expression in murine macrophages with or without dexamethasone treatment. Propidium iodide (PI) was used for nuclei staining. (E) Graph showing the normalized mean value obtained from MRI images of murine macrophages CD163 (+) and CD163 (−) incubated with NP-CD163(m) and NP-IgG(m), n = 3. All the replicates were normalized to the blank (cells that have not been incubated with any nanoparticles). The error bars shows the SEM. $$p < 0.01 versus Blank; *p < 0.05 and **p < 0.01 versus NP-CD163(+); #p < 0.05 versus Ab 1:1. F) Representative MRI phantoms of murine macrophages CD163 (+) and CD163 (−) incubated with NP-CD163(m) and NP-IgG(m).