Table 2 In vitro oxidation of methionine residues from therapeutic proteins.

From: Sulphur Atoms from Methionines Interacting with Aromatic Residues Are Less Prone to Oxidation

Protein PDB Low High TP TN FP FN Ref
Granulocyte Colony-Stimulating Factor 1CD9 122 127,138 1 2 0 0 8
Alpha1-Antitrypsin 1HP7 63,220,221,242,374,385 226,351,358 5 3 0 1 7
Prion Protein 1B10 154,206,213 129,134 2 2 0 1 53
IgG1-Fc 1FC1 358,428 252 2 1 0 0 54
Stem Cell Factor 1EXZ 36,48 27 2 1 0 0 55
Coagulation Factor VIIa 1QFK 327,391 298,306 2 1 1 0 56
Growth Hormone 1HGU 170 14,125 1 2 0 0 57
Chorionic Somatomammotropin 1Z7C 14,96,170 125,179 2 1 1 1 57
  1. The columns Low and High provide the positions of the methionines for which low and high reactivity, respectively, have been experimentally established. Whenever a methionine participated in an S-aromatic motif, it was predicted to have a low reactivity. Otherwise, the methionine was predicted to have a high reactivity. The numbers of true positive (TP), true negative (TN), false positive (FP) and false negative (FN) results are shown.