Figure 3 | Scientific Reports

Figure 3

From: Manual control of catalytic reactions: Reactions by an apoenzyme gel and a cofactor gel

Figure 3

Expression of catalytic activity via adhesion of the apoHRP gel to the FePor gel.

(a) Chemical structure of ABTS and its oxidation product (ABTS+•). (b) UV-Vis spectra of the oxidation of 0.5 mM ABTS in the presence of 1.0 μM apoHRP (blue diamonds), FePor (black dots) and the apoHRP/FePor complex (red squares) at 10 °C. (c) UV-Vis spectra of the oxidation of 0.5 mM ABTS in the presence of apoHRP (1.0) gel (blue diamonds), FePor (1.0) gel (black dots), blank gel (green triangles), the apoHRP (1.0) gel/FePor complex (red squares) and the apoHRP/FePor (1.0) gel complex (purple dots) at 10 °C. (d) Investigation of catalytic activity via adhesion of the apoHRP (1.0) gel/FePor (1.0) gel. (e) UV-Vis spectra of the oxidation of 0.5 mM ABTS in the presence of a combination of apoHRP (1.0) gel/blank gel (blue diamonds), blank gel/FePor (1.0) gel (black dots) and the apoHRP (1.0) gel/FePor (1.0) gel assembly (red squares). (f) Schematic illustration of the oxidation of ABTS via adhesion of the apoHRP gel to the FePor gel.

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