Table 2 List of antibodies used for immunofluorescence imaging.

From: Human Induced Pluripotent Stem Cell Derived Neuronal Cells Cultured on Chemically-Defined Hydrogels for Sensitive In Vitro Detection of Botulinum Neurotoxin

Primary Antibody Vendor Catalog #
1:500 Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein (GFAP) DAKO Z033401-2
1:500 Mouse Anti-Neuron-specific β3-Tubulin MAb (Clone TuJ-1) R&D Systems MAB1195
1:200 Mouse Anti-Anti-MAP2 Antibody, clone AP20 Millipore MAB3418
1:500 Rabbit Anti-GABA Abcam ab43865
1:50-1:100 Rabbit Anti-VGLUT2 Abcam ab84103
1:250 Polyclonal rabbit antibody, Synaptotagmin 2, cytoplasmic domain (SYT2) Synaptic Systems 105 123
1:100 Rabbit Anti-Glutamate Decarboxylase 65 & 67 Antibody (GAD 65/67) EMD Millipore AB1511
1:100 Synaptophysin (D35E4) XP® Rabbit mAb #5461 Cell Signaling 5461S
1:100 Synapsin-1 (D12G5) XP® Rabbit mAb #5297 Cell Signaling 5297S
1:100 Mouse anti-MNR2/HB9/Mnx1 DSHB 81.5C10
Secondary Antibody Vendor Catalog #
1:200 Alexa Fluor® 568 Donkey Anti-Mouse IgG Life Technologies A10037
1:200 Alexa Fluor® 568 Donkey Anti-Rabbit IgG Antibody Life Technologies A10042
1:200 Alexa Fluor® 488 Donkey Anti-Mouse IgG (H+L) Antibody Life Technologies A-21202
1:200 Alexa Fluor® 488 Donkey Anti-Rabbit IgG (H+L) Antibody Life Technologies A-21206