iLMO2 can be expressed in multiple structures of the basal ganglia and is able to induce ipsilateral rotation in awake rats.
(a) Intravenous administration of CTZ was able to significantly increase net ipsilateral rotations over mean baseline rate in rats unilaterally expressing iLMO2 in the striatum and globus pallidus. CTZ or vehicle was injected through a jugular venous catheter at t = 0 (dashed line). The response to CTZ was significantly different than vehicle by two-way ANOVA with Bonferroni posthoc test (*p < 0.05; n = 5 trials for CTZ, n = 6 trials for vehicle across 3 animals). (b) Expression of iLMO2 (green) was found throughout the entire striatum and globus pallidus (STR-striatum, GP-globus pallidus is outlined). Neurons in the globus pallidus expressed iLMO2 (bottom right) and sent labeled projections to the subthalamic nucleus (STN, bottom left). Blue: DAPI. (c) Bioluminescence image of a rat expressing iLMO2 after CTZ was administered intravenously under anesthesia. (d) Bioluminescence image of an acute brain slice showing activation of iLMO2 throughout the striatum and globus pallidus after addition of CTZ. Color bar indicates relative luminescence intensity.