MPTP-induced loss of TH-positive fibers in CPu and of TH-positive neurons in SNc.
(a) Photomicrographs of TH-immunostained sections from the striatum (CPu) of control (Ctrl, upper) and MPTP-treated (MPTP, lower) mice. Scale bar, 1 mm. (b) Immunofluorescent staining of TH (green) and NeuN (red) in slices from SNc (left) and VTA (right) of control and MPTP mice. Scale bar, 100 μm. (c) Normalized optical densities for TH-immunoreactivity in CPu of control and MPTP mice. The optical density of TH-immunoreactivity was normalized by the average optical density from control mice in each batch. ***P < 0.002. (d,e) The number of neurons expressing TH in SNc (d) and VTA (e) of control and MPTP mice. *P < 0.05. (c–e) nctrl = 6 mice, nMPTP = 6 mice.