Eco-friendly carbon-nanodot-based fluorescent paints for advanced photocatalytic systems

Fluorescent carbon nanomaterials, especially zero-dimensional (0D) carbon nanodots (CDs), are widely used in broad biological and optoelectronic applications. CDs have unique characteristics such as strong fluorescence, biocompatibility, sun-light response, and capability of mass-production. Beyond the previous green CD obtained from harmful natural substances, we report a new type of fluid-based fluorescent CD paints (C-paints) derived from polyethylene glycol (PEG; via simple ultrasound irradiation at room temperatures) and produced in quantum yields of up to ~14%. Additionally, C-paints possess a strong, UV- and visible-light-responsive photoluminescent (PL) property. Most especially, C-paints, by incorporation into a photocatalytic system, show additional roles in the emission of fluorescent light for activation of TiO2 nanoparticles (NPs) and the resultant detoxification of most organic dyes, thus further enabling embarkation in advanced water purification.


Results
Morphological structures and size distribution. The C-paints were assessed in their morphology and size distribution using transmission electron microscopy (TEM) and atomic force microscopy (AFM). TEM images show that these C-paints are uniform in size ranging from 2 to 6 nm in diameter. AFM images further confirm the uniformity of the C-paints. The height of the C-paints ranges from 1 to 8 nm (overall average: ~4.3 nm), without aggregation ( Fig. 1a and Supplementary Fig. 1).
X-ray diffraction (XRD) patterns. The C-paints were shown to be highly water-soluble as well as particle-aggregation-resistant over several days. The C-paints' XRD patterns showed broad peaks at 2θ = 21.9° and a weak peak at 2θ = 43.4°, which were assignable to the (002) and (101) diffraction patterns of graphitic carbon, respectively (Supplementary Fig. 2) 23 . These peaks revealed what was almost an amorphous carbon phase, which was attributed to the introduction of oxygen-containing groups by physico-chemical reaction 24 . The former peak corresponds to the interlayer spacing of ~3.8 Å, which is slightly longer than that between the (002) planes in bulk graphite (~3.5 Å) 23,24 . Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectra (XPS) analysis. FT-IR spectra were used to investigate the bonding composition and functional groups for the sample of as-synthesized C-paints at different synthesis time ( Supplementary Fig. 3). The peak at 1130 cm −1 is assigned to a -CH 2 stretching vibration [23][24][25] . The absorption at 2850-3000 cm −1 is attributed to C-H stretching vibration. The broad peaks at 3200-3600 cm −1 indicates O-H bonding vibration 24 , which results in the hydrophilicity of C-paints and improves their stability and dispersibility in water, without any further surface passivation 23 . The chemical bonding states of the functional carbon and oxygen groups of the C-paints were examined by XPS analysis. The XPS spectra exhibited three peaks between 284.6 and 530.6 eV, which arises from C1s and O1s, respectively. The atomic ratio of carbon to oxygen is 2.14. A high-resolution scan of the C1s region showed deconvolution of the C1s peaks present in the carbon atoms' different functional groups [-C-C or -C-H (284.6 eV), -C-O(H) (286.2 eV), and C-O-C=O (287.3 eV) peaks in the C1s spectrum], as evidenced in Supplementary Fig. 4 and Supplementary Tables 1 and 2 24,26 . These functional groups signify the solubility of C-paints in water without further chemical modification. All of the surface components of the C-paints measured using XPS are in good agreement with the FT-IR spectral results. The presence of oxygen-containing functional groups on the surface of amorphous C-paints can increase the surface properties along with the potential for photocatalytic and other flexible optoelectronics applications.
Optical properties of C-paints. As shown in the UV-Vis spectra in Supplementary Fig. 5, C-paints have one strong absorption band centered at 245 nm and the other broad band found in the visible range 27 . Upon excitation wavelength at 245 nm, bright fluorescence centered at 400 and 500 nm could be observed. Additionally, the emission intensity of the C-paints gradually increased as synthesis time increased. When the PEG polymer was treated by ultrasound irradiation for more than 1 hr, shoulder peaks began to appear in the PL emission spectra, as shown in Fig. 1b. The C-paints, compared with the original polymer, exhibited a sharply increased fluorescence under UV-as well as visible-light. Their emission spectra shifted from UV-to visible-light with the changes in the excitation conditions. Correspondingly, the emission in blue or red was observed at the wavelength ranging from 290 nm to 570 nm, which is a common phenomenon of reported CDs. The different-sized NPs (quantum effect) and/or emissive trap sites on the CD surface or another various mechanisms have been studied in order to establish the multicolor PL dependent on excitation wavelengths 9,28,29 . As seen in Supplementary Fig.  6, the PL spectra of the C-paints were gradually increased over the course of 4 hrs under excitation of 350 nm. Also, it is demonstrated the high photostability of C-paints for 7 days, that is PL properties of C-paints-4 sample (x 50 dilution) remains unchanged after storing at least for one month in air at room Scientific RepoRts | 5:12420 | DOi: 10.1038/srep12420 temperature. The quantum yield of C-paints was increased from ~1% to ~14% with increasing ultrasound irradiation time. On the basis of equation (see Methods) it can be calculated the quantum yield of C-paints. The quantum yield (QY) based on the equation (1) on these C-paints with tubular form is summarized in Supplementary Table 3. The strongest fluorescent emission with the enhanced maximum quantum yield of ~14% was observed at 370 nm and centered at 450 nm. In the CDs's formation process, fluorescent C-paints become softer and stickier properties than pure PEG. Ultrasound irradiation at room temperature with no additional solvent removes residues from PEG polymer and endows C-paints with adhesive characteristics, which leads to C-paints attachable to various substrates. This sticky effect is more pronounced to the naked eye when C-paints are "spread" on aluminum foil or paper (Fig. 1c,d).

Biocompatibility of C-paints. An important characteristic of fluorescent materials with respect
to biological and environmental applications is biocompatibility 23,24 . Its cytotoxicity was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay using the CHO-K1 (ovary, Chinese hamster), COS-7 (kidney, African green monkey) and HeLa (cervical cancer cells, human) cell lines. Cells with C-paints concentrations ranging from 0 to 10 mg/mL were incubated for 24 hrs 23 . As shown in Fig. 2, the C-paint showed no inherent cytotoxicity to the CHO-K1, COS-7 or HeLa cells (cellular viability: 97% ± 3% for CHO-K1, 94% ± 5% for COS-7, and 96% ± 3% for HeLa cells) when the amount of C-paints less than 5 mg/mL was used. At higher concentration of C-paints (5 mg/mL), the cell viability was over 95%, indicating good biocompatibility with low-or non-cytotoxicity. Beyond that concentration, the cell viability was slowly decreased in a concentration-dependent manner. Further cytotoxicity testing in animal cells is carefully considered to ensure the environmental safety of C-paints. Photocatalytic performances under UV-and visible light. Photocatalysis with sun-light has the potential to be a valuable technology for degradation of harmful organic compounds. For photocatalytic applications, the fraction of UV-light available is very low; therefore, visible light-responsive photocatalysts or additional light sources are needed. As an alternative offer, the use of C-paints as a fluorescent source, for example, can increase the photodegradation activity of TiO 2 NPs. Based on the FT-IR spectra obtained in the present study, the C-paints exhibited very good solubility in water, due to the presence, in the aqueous photocatalytic systems, of abundant hydrophilic groups that can freely disperse in water for reception and delivery of outer UV-and visible-light.
The photocatalytic activity and stability of the C-paints, UV-responsive commercial TiO 2 (P25) and visible-light-responsive 1%-Mn-doped TiO 2 (Mn-TiO 2 ) NPs were evaluated for the degradation of the organic dyes reactive black 5 (RB5) and rhodamine B (Rho B) under UV-and visible-light irradiation 30 . Prior to irradiation, the mixed solution of C-paints, TiO 2 NPs and organic dyes in aqueous solution was stirred in the dark for 30 min to confirm the adsorption/desorption equilibrium condition (A30). Compared with bare TiO 2 NPs (commercial P25 and Mn-TiO 2 ), TiO 2 NPs with C-paints exhibited enhanced photocatalytic efficiencies. Figure 3 indicates that the rates of photodegradation of organic dyes for TiO 2 NPs with C-paints were two-fold higher than for pure TiO 2 (P25) NPs and three-fold higher than for Mn-TiO 2 NPs under exciting irradiation. These results show that C-paints can remarkably improve photocatalytic efficiency in the bulk solution. Our finding indicates that the degradation rate was gradually increased with increasing content of C-paints or TiO 2 NPs in comparison to the control samples ( Supplementary Fig. 7).
In photocatalytic process of TiO 2 NPs, C-paints can, as shown in Fig. 4, enhance the amount of light available for TiO 2 NPs, thereby accelerating photocatalytic TiO 2 reaction. First, in the shallow region of the dye solution, TiO 2 NPs exhibited effective photocatalytic activity under close-by lamp irradiation, as the transparent C-paints facilitated the absorption of light into the TiO 2 NPs NPs. As the light source proceeded towards the perpendicular direction, the light-absorption distance gradually decreased, showing the lowest light absorption at the bottom of the dye solution. However, when C-paints are illuminated, they absorb UV-and visible-light and then emit UV-and visible-light energies, which in turn excites in TiO 2 and Mn-TiO 2 NPs again. This is expected to enhance the photo-response of TiO 2 NPs to form electron-hole pairs. Certainly, C-paints are expected to be a unique material for enabling immediate delivery of light into the surrounding microenvironments of Mn-TiO 2 NPs either isotropically or multi-directionally. To further demonstrate the photocatalytic stability of the C-paints, recycling tests of RB 5 degradation with TiO 2 (P25) were performed. As shown in Supplementary Fig. 8, after 10 recycles for the degradation of RB 5, the photocatalytic activity of the C-paints with TiO 2 system presents about 15% loss. It is associated that loss of TiO 2 NPs was occurred during washing and drying for reuse. In a particular photocatalytic process, the separation of small-sized TiO 2 NPs from suspended solution could be a hard work for the purpose in recycling uses 30 .

Discussion
The concept of C-paints as multi-directional light sources has been demonstrated by using TiO 2 and Mn-TiO 2 NPs as an photocatalytic system for simultaneous light adsorption and delivery. The fabricated C-paints with TiO 2 NPs samples showed much increased photodegradation efficiency. C-paint enhances   These results open new perspectives for fluid based mobile light sources. Importantly, C-paints showed high quantum yields (~14%), water solubility and very low cytotoxicity against CHO-K1, COS-7 and HeLa cells. for environmental application.
In summary, treating bare PEG polymer under ultrasound irradiation was proven to be a facile method for large-scale preparation of C-paints. These C-paints are easy to produce at room temperature and can be excited by UV-as well as visible-light. We further demonstrated the use of such C-paints media as high-fluorescent and water-soluble media for an innovative photocatalytic system, finding that designed complex photocatalysts (C-paints/TiO 2 NPs) efficiently enhanced photocatalytic efficiencies. In this system, C-paints as mobile light sources have obvious advantages in adjusting the decomposition of organic materials by the emitting fluorescence throughout the surface of TiO 2 NPs in water, which increased light-absorbing surface area of TiO 2 NPs. Fluorescent C-paints as advanced concept of fluid type light are expected to be utilized effectively and widely in biological processes and flexible optoelectronics.

Methods
Fabrication of C-paints. Polyethylene glycol (PEG) (average Mn = 300, Sigma-Aldrich, MO, USA) as a biocompatible non-conjugated polymer was used as CDs source. For the one-step synthesis of C-paints, 40 mL PEG was placed in a glass bottle (57 mm in width × 108 mm in height) as a carbon source was treated with ultrasound irradiation at room temperature temperature (frequency = 40 kHz) for 1, 2, 3 and 4 hrs, respectively. The whole experiment was worked on without additional solvents. As the ultrasound irradiation time increased, the solution changed from colorless to yellow brown. At last, 40 mL brown fluid containing carbon nanoparticles was obtained and named C-paint-n (where n indicates synthesis time). We determine the synthetic conditions (1-4 hrs) under the optimized non-toxic state (< 5 hrs; < 5 mg/ml), because we attempted to improve eco-friendly photocatalytic efficiency using C-paints.
Sample characterization. The morphological structure and size of the C-paints was analyzed by high-resolution transmittance electron microscopy (HRTEM) and atomic force microscopy (AFM, VEECO Instrument, USA). The HRTEM specimens were prepared by drop-casting 10 μ L of the C-paints solution on a 300 mesh carbon-coated copper TEM grid with a carbon film, followed by drying at room temperature. For the AFM analysis, 100 mL of the C-paints was placed on a silicon wafer. The wafer was air-dried for 24 hrs, and the remaining solution was dispersed using an air gun. In a corresponding particle-size-distribution histogram, the C-paints were plotted, based on a nanoparticle count. UV/Vis absorption spectra were recorded by a UV-Vis-NIR spectrophotometer (Varian, Cary 5000, Australia). Photoluminescence (PL) spectra were recorded using a UV transilluminator (DUT-260; Core Bio System, Korea) to measure the optical properties of C-paints. The excitation wavelengths were 290-570 nm. The internal quantum yield was measured with the Quantum Yield System (K-MAC, Fluoro-Q2100) at 370 nm excitation by the equation: where Ec is the emission produced by direct excitation light, La is the total amount of excitation light and Lc is the amount of light after direct excitation 31 . The reference solution used was water. Transmission Fourier transform infrared (FT-IR) spectra were acquired using a JASCO FTIR 470. Each spectrum was recorded from 4000 to 900 cm −1 in 12 scans at a resolution of 4 cm −1 . High-resolution X-ray photoelectron spectroscopy (HR-XPS) was carried out using monochromatic Al Kα X-ray radiation (hν = 1486.6 eV) with a power of 120 W (Kratos Analytical, AXIS Nova, UK) to investigate the surface properties of the samples. The shift in the binding energy due to relative surface charging was corrected using the C1s level at 284.6 eV as an internal standard. Depth-profiling XPS with Ar + ion bombardment was performed.
Biocompatibility. CHO Photocatalytic properties. The rhodamine B (Rho B; 3 mg/L, pH 5.5, Sigma-Aldrich, USA) and reactive black 5 (RB 5; 3 mg/L, pH 6.67, Sigma-Aldrich, USA) solutions were placed in test reactor, and the dye was adsorbed in C-paints under the dark for 30 min (A30). After the adsorption process of the dye was completed, C-paints (0.5-5.0 mg/mL) were dissolved in dye solution and photocatalyst samples (TiO 2 and Mn-TiO 2 NPs) were added to the final solution. The photocatalytic degradation of the Rho B or RB 5 solution with catalyst samples (0.5 g/L) and C-paints (0.5-5.0 mg/mL) was carried out under UV-(source: 4 W, 365 nm, VSLAB VL-4CL, Korea) and visible-light (source: 150 W Xe lamp, λ > 420 nm, SCHOTT, USA) irradiation 32,33 and the absorbance of the solutions was measured using a UV-Vis-NIR spectrophotometer (Varian, Cary 5000, Australia) in the wavelength region of 200-800 nm. The post-photo-irradiation concentrations of RB 5 and Rho B in the filtered supernatant solutions were measured from the absorbance peak intensities of the solutions at 598 and 555 nm, respectively 33,34 . The changes in the dye-solution concentration [ln(C 0 /C) = kt, where k is the apparent reaction rate constant, and C 0 and C are the initial and reaction concentrations at time = 0 and time = t, respectively, of Rho B] also were investigated. To investigate the repeatability of the C-paints complex photocatalytic system, photocatalytic experiments were repeated. After reactions, the TiO 2 NPs were collected by centrifugation (6000 rpm, 10 min). The separated TiO 2 NPs was performed washing with distilled (DI) water and drying in an oven (65 °C) for 3 hrs after every cycle. The treated TiO 2 NPs was reused in the photocatalytic reaction for 10 times under the identical C-paints and light irradiation conditions.