Figure 3 : Chemical family classification-based screening of anti-inflammatory compounds from CP.

From: A strategy for the identification of combinatorial bioactive compounds contributing to the holistic effect of herbal medicines

Figure 3

Cells were pretreated with tested samples (at a corresponding concentration in 0.4 mg/ml CP) for 1 h, followed by treatment with LPS (1 μg/ml) and incubation for indicated time. Concentration of (a) NO, (b) PGE2 and (c) IL-6 in the supernatant. (d) Protein expression levels of iNOS and COX-2. β-actin was used as an internal loading control. The expression levels of mRNA for (e) iNOS, (f) COX-2, (g) IL-6 and (h) IL-1β were analyzed by quantitative real-time PCR. GAPDH served as internal control for normalization of mRNA expression. Data are presented as mean ± SD of three independent experiments performed in duplicate. ##p < 0.01 versus control group; *p < 0.05, **p < 0.01 versus LPS group (one-way ANOVA, Dunnett test). CP: Cardiotonic Pill; PA: combination of 10 phenolic acids; GN: combination of 4 ginsenosides; TN: combination of 4 tanshinones; BECCs: bioactive equivalent combinatorial components (combination of PA, GN and TN).