Figure 4 : Quantification of cellular death and antifungal activity of mast cells.

From: Opportunistic pathogen Candida albicans elicits a temporal response in primary human mast cells

Figure 4

(A) The graph depicts the relative amount of MCETs per micrograph of C. albicans-infected mast cells (MOI 0.1) as compared to the uninfected mast cell control at two different time points. At both time points analysed (6 h and 10 h) the variation between MCETs compared to uninfected samples was analysed for statistical significance. (B) The graph represents the viability of fungal cells after normalization to the biological replicates 100% growth control. C. albicans viability is reduced in mast cell infection (MOI 1) up to 3 h. (C) C. albicans-induced mast cell death in a time and dose-dependent manner as determined with Sytox green. The Y-axis represents the relative amount of dead cells after normalization to the mast cell lysis control. (A) Significance was analysed by t-test and by Tukey one-way ANOVA (B) *P ≤ 0.05. (B) Data are presented as means of at least six replicates (A) and n = 3 (6) ± SD. (C) For cell death significance was analysed by Bonferroni two-way ANOVA *P ≤ 0.05 comparing to the mast cell uninfected control at each time point. Data represents n= 4 (5) ±SD.