Figure 2 : Neutrophils but not monocytes differentially migrated in response to supernatants from mast cells infected with C. albicans.

From: Opportunistic pathogen Candida albicans elicits a temporal response in primary human mast cells

Figure 2

Supernatants collected from C. albicans-infected mast cells (MOI 0.1) at 6 h, 12 h and overnight infection were used as chemoattractants to neutrophils and monocytes in a transwell system. End-point cell migration was plotted per condition, per time as ratio of migrated cells using as 100% control cells added to the lower compartment without inserted transwell system. (A) Neutrophil migration is increased over time towards supernatants of infection but not to C. albicans and HMC-1 alone (controls). (B) Monocytes show no significant chemoattraction towards supernatants of infected mast cells. Variations between neutrophil or monocyte migration over time towards supernatants of infection and C. albicans control were analysed for statistical significance using a one-way ANOVA with Tukey post-test. As positive control for migration we used fMLP causing chemotaxis significantly above background of approximately 45% after 30 min for neutrophils and 13% after 90 min for monocytes. These values are indicated as a horizontal, dashed line in the graphs of the figure. Data are presented as means of n = 5 (3) ± SD.