Figure 4 : Developmental profiles of BmE75 in prothoracic glands.

From: Bombyx E75 isoforms display stage- and tissue-specific responses to 20-hydroxyecdysone

Figure 4

(A-A”) Developmental profiles of mRNA levels of BmE75A (A), BmE75B (A’) and BmE75C (A”) in the prothoracic glands from day 2 of the 5th larval instar (L5D2) to day 2 of the prepupal stage (PP2). L, instar; D, day; EW, early wandering; LW, late wandering; PP, prepupal; P, pupa. (B-B’) Developmental profiles of protein levels of BmE75A, BmE75B and BmE75C in the prothoracic glands from L5D2 to PP2. The full-length blots are presented in Supplementary Figure 1. (B’) Quantification of BmE75A, BmE75B and BmE75C protein levels in (B). Developmental profile of ecdysteroid titer in the hemolymph measured by radioimmunoassay from L5D2 to PP2.