Figure 2 | Scientific Reports

Figure 2

From: Cyclic AMP Mimics the Anti-ageing Effects of Calorie Restriction by Up-Regulating Sirtuin

Figure 2

cAMP Treatment Improves Adipose Metabolism to Mimic Calorie Restriction.

(A) The curve for the body weight gain. (B) Daily food intake. (C) Latency to fall from a rota-rod indicated the motor coordination of mice. (D) The photographs of epididymal adipose tissue. (E) Histological analysis of liver tissue, which was stained with oil red O to visualize lipid content and counterstained with haematoxylin. (F) Abdominal adipose tissue stained with haematoxylin and eosin. (G) The total cholesterol in mouse liver. (H) The total glycerides in mouse liver. (I) Western blot results for the PKAc, PEPCK, PPARγ, CaMKKII, SIRT1 and SIRT3 protein levels in treated or untreated mouse liver. The gels were run under the same experimental conditions. (J) Fold change in Adiponectin levels in liver. (K) MDA level in mice liver. All the statistical analyses were performed using one-way ANOVA. #p < 0.05, ##p < 0.01 compared with control mice; *p < 0.05, **p <0.01, ***p < 0.001 compared with mice fed a high-fat diet.

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