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Figure 1

From: Cyclic AMP Mimics the Anti-ageing Effects of Calorie Restriction by Up-Regulating Sirtuin

Figure 1

cAMP Treatment Mimics the Calorie Restriction Effect to Improve Ageing-associated Phenotypes in Aged Mice.

(A) The appearance of aged mice (24-month-old) with (right) or without (left) db-cAMP treatment. The red line indicates the angle of the mouse spine. (B) The survival curve of aged mice with (red line) or without (black line) db-cAMP treatment. The red square indicated the administration period (12 weeks after 24-month-old) (n = 18–20 mice per group). (C) Morris water maze test result for mice with (red line) or without (black line) db-cAMP treatment. (D) Open field test result indicating the local motor activity. The data were captured by automatic detection. (E) Latency to fall from a rota-rod indicated the motor coordination of mice. (F) Latency to fall from a string indicated the muscle strength of mice. (G) HE staining for the liver of mice. The arrow indicates the hydropic degeneration phenotype. (H) HE and Masson’s staining for the skin of mice. The arrow indicates the thickness of collagen or muscle. Western blot results for the p-AMPK (Thr172) protein level in mice with or without calorie restriction treatment. (I,J) Protein carbonyl and MDA levels in liver tissue. (K–M) Fold change of cAMP level in mouse liver, muscle and heart with CR treatment. (N) Western blot results for PKAc, CaMKKII, SIRT1 and SIRT3 protein levels in the liver, with or without db-cAMP treatment (20 mg/kg db-cAMP). The gels were run under the same experimental conditions. (O) Western blot results for PKAc, CaMKKII, SIRT1 and SIRT3 protein levels in the liver, with or without calorie restriction treatment. The gels were run under the same experimental conditions. All the statistical analyses were performed using one-way ANOVA. #p < 0.05, ##p < 0.01 ###p < 0.001 compared with untreated young mice; *p < 0.05, **p < 0.01, ***p < 0.001 compared with untreated aged mice.

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