Figure 8 : TVs interact with Maackia amurensis leukoagglutinin (MAL).

From: Tulane virus recognizes sialic acids as cellular receptors

Figure 8

CsCl gradient-purified TV (1 × 104 PFU/mL) was coated on a microtiter plate and incubated with biotinylated MAL and Sambucus nigra lectin (SNL). Detection of bound biotinylated MAL/SNL by streptavidin-horse radish peroxidase (HRP) conjugates through ELISA showed interaction between TV and MAL (TV-MAL and TV-MAL long), but not SNL (TV-SNL). Coating the same gradient fraction of uninfected LLC-MK2 cell lysate and incubation with MAL revealed no signal (Cell lysate-MAL, OD <0.1) that serves as a negative control. Incubation of purified TV with MAL for longer time (overnight instead of one hour in the other experiments) revealed higher binding signals (TV-MAL long). A dashed line indicates the signal cutoff of 0.1. Each experiment was repeated at least four times. The statistical differences between data groups are indicated.