Figure 6 : Histo-blood group antigen (HBGA) expression and Tulane virus (TV) infection of LLC-MK2 cells.

From: Tulane virus recognizes sialic acids as cellular receptors

Figure 6

The HBGA expression (green) of LLC-MK2 cells was determined through immunostaining using a panel of monoclonal antibodies (Mabs) against various HBGAs, which revealed only B antigen expression. Replicating TV virions (red) were detected by in-house hyperimmune rabbit serum against TV. Nuclei were stained with 4’,6-diamidino-2- phenylindole (DAPI, blue). (A to D) LLC-MK2 cells at 20 hours post-TV infection were stained using Mabs against B antigen (green) and hyperimmune serum against TV (red). Some B antigen-expressing cells were not infected with TV (A, B and D), while some TV-infected cells lacked detectable B antigen expression (B, C and D). (E and F) Cells were stained using the same procedure without the primary antibodies against B antigen and TV as negative controls.