Figure 4 : The enhancement effects of Maackia amurensis leukoagglutinin (MAL) on TV infectivity are abolished by neuraminidase treatment.

From: Tulane virus recognizes sialic acids as cellular receptors

Figure 4

LLC-MK2 cells were cultured in 12-well plates for plaque assays. Treatment of cells with Vibrio cholerae neuraminidase (VCNA, 60 mU/well) inhibits, while MAL treatment (100 μg/mL) of mixed TV/LLC-MK2 cells increases TV infectivity. The enhancement effects of MAL disappeared when the cells were pre-treated with VCNA. The mean values (plaque numbers) of the mock/control group were set as 100%, while percentages (%) of the plaque numbers for individual wells of mock/control, VCNA-, MAL-, and VANA+MAL-treated groups relative to the mean values of the mock/control group were calculated and shown. Each experiment was repeated at least four times. The statistical differences between the mock/control groups and the VCNA-, and VANA+MAL-treated groups are indicated. “**” indicates P values < 0.01.