Figure 7 : NAC/AAP decreased the activation of Drp1 in H2O2-treated hADMSCs.

From: Synergistic Protection of N-Acetylcysteine and Ascorbic Acid 2-Phosphate on Human Mesenchymal Stem cells Against Mitoptosis, Necroptosis and Apoptosis

Figure 7

(A) The co-localization of Drp1 Ser616 (green) in the mitochondria (MitoTracker, red) was revealed by confocal microscopy. The merged images clearly show the recruitment of Drp1 Ser616 to mitochondria in response to H2O2 treatment. NAC/AAP pretreatment partially prevented mitochondrial translocation of Drp1 S616 induced by H2O2 in a time-dependent manner. The fluorescence data were quantified by ImageJ software. Quantification data were obtained from at least five independent experiments (lower panel). (B) Western blot analysis was employed to study the translocation of Drp1 to mitochondria in response to H2O2 treatment. The protein levels of Drp1 were decreased by Nec1 pre-incubation. Long exposure revealed ~160 and ~300 kDa bands in the mitochondrial fraction. Scale bar = 10 µm.