Effect of flow rate on data quality.
100 μM [Glu]-1 fibrinogen B, m/z 785.8 (doubly charged ion) in 50% acetonitrile, 0.1% formic acid in water was infused into the mass spectrometer via the SAWN interface. Data was averaged over 0.5 min. Flow rates were: (a) 1 μLmin-1, (b) 2 μLmin-1, (c) 5 μLmin-1, (d) 10 μLmin-1. As flow rate increases above 5 μLmin-1 the signal to noise decreases and additional peaks become apparent in the spectra. Signal to noise ratios for the different flow rates are shown in table 1.