A Photochromic Sensor Microchip for High-performance Multiplex Metal Ions Detection

Current multi-analytes chips are limited with requiring numbers of sensors, complex synthesis and compounds screen. It is expected to develop new principles and techniques to achieve high-performance multi-analytes testing with facile sensors. Here, we investigated the correlative multi-states properties of a photochromic sensor (spirooxazine), which is capable of a selective and cross-reactive sensor array for discriminated multi-analytes (11 metal ions) detection by just one sensing compound. The multi-testing sensor array performed in dark, ultraviolet or visual stimulation, corresponding to different molecular states of spirooxazine metal ions coordination. The facile photochromic microchip contributes a multi-states array sensing method, and will open new opportunities for the development of advanced discriminant analysis for complex analytes.

T here is an increasing need of convenient, rapid, sensitive and high-throughput sensors for target multianalytes identification in the environmental monitoring [1][2][3][4] , aliment safety 5,6 , clinical diagnoses [7][8][9][10][11][12][13][14] , and so on 15,16 . Sensor arrays are a verified valid detection method in the area of multi-analytes testing and highthroughput analysis [17][18][19][20] . Currently, a graphene-based ensemble aptamers were exploited to recognize molecular or cellular targets discriminatively 10 . Cui et al. developed a sensor array assembled from polyionic liquid inverse opaline microspheres to test five anions 16 . Generally, the sensor array performed the correlative differential analyzing needs large numbers of serial compounds or complicated compound with several recognition groups as sensor, which involves complicated chemical synthesizing and valid compounds screening. Examples include adopting six various 8-hydroxy-quinoline derivants to realize metal ions detection 21 , designing sensor combining four hydrophobic recognition groups to take molecular diagnostics 22 , seven sensors utilized a common calix [4] pyrrole receptor to detect 14 carboxylates 23 . Although the serial sensors own high sensitivity, the high production cost and challenges regarding valid compounds screen restrict their practical applications. These facts call for a reliable sensor to alleviate these concerns for general high-performance multi-analytes detection. Here, we adopt a photochromic material, spirooxazine, to multi-testing sensor array, and utilize the multi-states of the sensing array under stimulations acting the selective and cross-reactive sensing (Fig. 1). This photochromic sensor microchip can actualize eleven various metal ions detection with only one facile sensor, which offers a new strategy to design and develop high-performance multi-testing sensor array.
Spiropyran and spirooxazine (SP), which respond to different external stimulations, such as light, proton and metal ions to undergo reversible structural interconversion accompanied the change in photophysical properties, are of interest in the emerging area of logic gates and communication networks [24][25][26] , molecular switches and unit 27,28 , data storage 29 , metal ions detection and other fields [30][31][32][33][34][35][36][37] . With different light stimulations, spiropyran and spirooxazine interconvert among the closed form to merocyanine form (MC). The merocyanine spiropyran/ spirooxazine can combine with proton or metal cations to form metallic merocyanine (MMC), as shown in Fig. 2 [24][25][26][30][31][32][33][34][35][36][37] . Performing the reversible reactions between closed, MC and MMC forms of spiropyran/spirooxazine, different ions strength and light stimulations have the differential inductive effect. It gives abundant chemical information in forms of interconvert processing, which can be adopted to high-throughput and complex analysis. Since fluorescent detection owns the significant advantages of high-sensitive, quick-response and non-contacting, we choose a commercial spirooxazine: 1, 3, 3-trimethylindolinonaphthospirooxazine to build a photochromic sensor microchip for high-performance multiplex metal ions detection. convert to open or closed form. Furthermore, when this sensing system was adopted in cross-reactive array sensing, fluorescent signals were collected in the same condition at the same time, which will avoid error from excitation light source glint, detector fatigue. Accurate chemical difference was present in this sensing array sys-tem. The phenomena that spirooxazine-metallic coordinations reveal various fluorescent spectra in different light stimulations demonstrate the sensing molecular spirooxazine can provide multiple fluorescent signals, which will contribute to the photochromic sensor microchip selective and cross-reactive testing.

Results
Based on the fluorescent enhancement or shift of different spirooxazine-metallic coordination states, we designed a microchip adopting spirooxazine as the only one sensor, analysing the fluorescent signals to realize multiple metal ions detection. To generate the cross-reactive SP microchip, we carried out 1.0 mM 1, 3, 3-trimethylindolinonaphthospirooxazine and thermoplastic polyurethane TecoflexH (0.5% PU in Ethanol) solution (200 nL) pipetted onto each pixel of the microchip. Here we used PU as the interface medium for the sensing in each pixel. PU is a block polymer with terephthalic acid units and ethylene glycol units, which is solvable in ethanol and swellable in water. On this microchip, AlCl 3 , CaCl 2 , CdCl 2 , CoCl 2 , CrCl 3 , CuCl 2 , FeCl 2 , HgCl 2 , MgCl 2 , NiCl 2 and ZnCl 2 (1.0 mM in water, 200 nL, pH 5 5) and pH 5 5 control aqueous solutions (HCl-NaCl) were spotted on corresponding pixels in 12 rows respectively. The fluorescence responses of the photochromic spirooxazine microchip to the presence of 11 metal ions (Al 31 , Ca 21 , Cd 21 , Co 21 , Cr 31 , Cu 21 , Fe 21 , Hg 21 , Mg 21 , Ni 21 and Zn 21 ) were recorded in 6 channels (CH 1: 450 nm, CH 2: 480 nm, CH 3: 505 nm, CH 4: 535 nm, CH 5: 570 nm and CH 6: 605 nm, with UV 365 nm excitation). The colours were generated by   Fig. 4b displays obvious different fluorescence intensity and shift for each row, which will contribute more information for high-performance multi-testing.
The discriminatory capability of the photochromic SP microchip can be performed using multivariate analysis. Linear discriminant analysis (LDA) was used to evaluate the similarities between the data corresponding to the same cluster by introducing the group classification [38][39][40][41][42] . LDA provides a graphic representation useful to gain an insight into the clustering of the response data, and to calculate classification accuracy. Fig. 5a, b represent the LDA score plot of the first three dispersion factors (F1-F3) describing of the total variance, which displays the clear clustering of the data. Since LDA trains the data to describe the best-fit parameters to separate different clusters, the distance of the cluster in spatial distribution reveals differential fluorescent signal of the metal ions. Obviously, the LDA score plot shows clustering for all 12 samples (7 trials each, 1.0 mM). It gives the 100% correct clustering of the eleven metal ion samples and one control sample water pH 5 5 (Table S1, Supporting Information [SI]). The clusters were separated in the space of differential fluorescence peak (435 or 533 nm) distribution orientation. Furthermore, we also adopted hierarchical clustering analysis (HCA) to carrier out multivariate analysis. HCA performs dimensionality reduction analysis to investigate the similarity clustering of the analytes. In this work, we defined the cluster by Ward's (minimum variance) method 43 , which takes into consideration the minimum amount of variance between the samples. The HCA result shows a dendrogram of euclidean distance between 84 samples with ward linkage in Fig. 5c and Fig. S1. HCA graphical output displays three major groups, which indicates fluorescent variation trend of the different states of spirooxazine-metallic coordination. Overall evaluating the LDA and HCA, the results reflect the original information of sensing, which indicates subtle and accurate details for the photochromic SP microchip sensing various metal ions. The spirooxazine-metallic responses focus on the two major peaks of 435 and 533 nm on fluorescence spectra. The different fluorescence intensity ratios between 435 and 533 nm reveal various classification contributions of photochromic SP microchip in different light stimulations.

Discussion
To evaluate the classification efficiency of the photochromic SP microchip with various states, we explored the LDA of SP array in dark, UV or Vis stimulation state separately (Table S2- Table S5 displays the near and confused spatial distribution of the clusters in each single state of SP array, which reveals the small differential of fluorescent signals of the metal ions in these analysis systems. In details, the SP sensing array in dark state can discriminate Al 31     states are highly complementary in distinguishing metal ions. It guarantees the integrated SP multi-states array to perform the 100% correct clustering of the eleven metal ion samples with one control sample. The key point of sensor array successful in multi-targets analysis is to provide enough differential and correlative signals. However, most single sensing molecule lacks enough differential signals to correctly cluster of the multi-analytes. Previous researches usually focused on developing serial sensors to get correlatively differential messages [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] . The strategies usually are preparing sensor array with serial sensor compounds to plus and fold the correlative response difference, or synthesizing special and complex sensor compound to generate obviously discriminated response. Here, the single photochromic sensing compound gives a strong correlativity between the configurations interconverting under different light stimulations. The differential states under dark, UV and Vis stimulation generate differential and correlative signals, which perform as three serial sensors adopted in this sensing array and contribute to perform a correct discriminant analysis. By the well-known Kolmogorov's law of the iterated logarithm 44 , the relation of multi-targets analysis successful probability (P) and participant observation states (n i ) can be deduced as (For the detailed deduction process, please see Sup-Information): There are many factors contribute to the probability of success of multi-targets analysis, here we focus on the influence of n i . The x ij presents the classification of success. The mathematical deduction suggests the universality of the strategy applied in multi-targets analysis. It can be simply described as a monotonically increasing function. That is meaning the calculated probability of success obviously increases when the observation's number increases, which is well in agreement with experiment results. The multi-states analysis method guarantees the generated signals cross-reactive and differential, which realizes high-performed multi-analytes identification on a single sensing compound array, and avoids the complex process of serial synthesis and valid compound screening.
Encouraged by the results above, we explored the utility of the spirooxazine multi-states sensor array by exploring a potential application: identification of mineral and purified water (Milli-Q water) based on their cation content as shown in Fig. 6. Table 1 lists    Figure S6). The study demonstrates that the photochromic SP microchip detection of metal ions can be generated into metal ions mixtures. Furthermore, we also processed the photochromic SP microchip for detecting metal ions in human serum (protein removed), and the LDA result displays 100% classification accuracy (Fig. S2, Table S7). The result suggests its potential applications in complex physiological environment.
In conclusion, utilizing interconversion of photochromic materials, we developed a high-performance multi-analytes sensor array with a single compound. Significantly, this sensor array is capable of identification of 11 metal ions, based on multi-states of spirooxazine in UV and Vis irradiations. The successful discriminating of various natural mineral water samples proves the photochromic SP microchip practical application in mixed and complicated analysis. It contributes a new idea of stimulated (photo-, electro-, magnet-, thermo-, chemo-, etc) conversion sensor array for multi-testing, which will have implicative applications in chemical, biological or medicinal samples testing. The future complex sample detections for pollution monitoring, aliment safety and clinical diagnoses will be facile, efficiency and low-cost with the high-performance sensing and analysis method.

Methods
Fluorescence testing. A UV LED lamp with the wavelength of 245 nm was used as the UV illumination source. Fluorescence measurements were performed in a Hitachi F-4500 Fluorescence Spectrophotometer. The absorption spectra were measured with a fiber optic UV-Vis-IR spectrometer (Ocean Optic HR 4000 CG).
Data analysis. The statistical multivariate methods, LDA and HCA [38][39][40][41][42] are routinely used to interpret and evaluate the responses from cross-reactive sensor arrays, providing a graphical output useful to gain an insight into the clustering of the response data, and calculate classification accuracy. The metal ion spots were formed on microchip and record in six channels by fluorescence scanner. The LDA was carried out using SYSTATH v12.02.00 and the HCA was carried out using MinitabH v16.1.1.0.