Figure 3 : Neighbor-joining diagram of 1,211 COI sequences produced from Illumina MiSeq sequencing of 1,010 individual arthropods.

From: Massively parallel multiplex DNA sequencing for specimen identification using an Illumina MiSeq platform

Figure 3

Distance measurement is calculated in number of base substitutions per site based on the Kimura 2-parameter method. Sequences originating from individuals morphologically identified as Coleoptera (blue), Psocoptera (red), and Trombidiformes (green) are indicated. Distinction is also made between sequences that correctly matched morphology based on a BLAST comparison to public COI databases (outlined), and those that did not match morphology (filled in). Individuals producing a single sequence are depicted as circles, whereas multiple sequences from the same individuals are depicted with triangles.