Irisin – a myth rather than an exercise-inducible myokine

The myokine irisin is supposed to be cleaved from a transmembrane precursor, FNDC5 (fibronectin type III domain containing 5), and to mediate beneficial effects of exercise on human metabolism. However, evidence for irisin circulating in blood is largely based on commercial ELISA kits which are based on polyclonal antibodies (pAbs) not previously tested for cross-reacting serum proteins. We have analyzed four commercial pAbs by Western blotting, which revealed prominent cross-reactivity with non-specific proteins in human and animal sera. Using recombinant glycosylated and non-glycosylated irisin as positive controls, we found no immune-reactive bands of the expected size in any biological samples. A FNDC5 signature was identified at ~20 kDa by mass spectrometry in human serum but was not detected by the commercial pAbs tested. Our results call into question all previous data obtained with commercial ELISA kits for irisin, and provide evidence against a physiological role for irisin in humans and other species.

I n 2012, Boström et al. 1 described irisin as a cleaved and secreted part of the transmembrane protein FNDC5 (fibronectin type III domain containing 5). They proposed irisin as an exercise-induced myokine triggering ''browning'' of white adipose tissue. These findings sparked a debate mainly turning on two issues: (I) the relevance of irisin in humans, (II) the specificity of commercially available enzyme-linked immunosorbent assays (ELISA), and more specifically the polyclonal antibodies on which they were based.
First, following the initial study 1 , it was realized that the start codon of the human FNDC5 gene is mutated from the normal ATG to ATA. There are examples of proteins being expressed from unusual start codons 2 , however, Raschke et al. 3 found that FNDC5 transcripts derived from the AUA start codon were translated to protein with extremely low efficiency as compared to the normal AUG start codon. All other animal species have an ATG as start codon at this position. This suggests that the human species has an effective gene knockout of FNDC5 and, consequently of irisin. Furthermore, Timmons et al. 4 expressed doubts about the response of FNDC5 mRNA in human muscle to exercise, based on their previous and larger data sets, which showed no such response. Nevertheless, a number of research groups around the world have examined the effects of exercise on irisin levels in human serum. These studies, mostly using commercial ELISA kits that are questioned here, have given contradictory results. Huh et al. 5 observed a significant increase in serum irisin in response to acute exercise after 1 week of moderate training whereas no effect of acute exercise was found after 8 weeks of training intervention. Short term effects of exercise on irisin levels were also reported by some authors [5][6][7][8][9] . In contrast, no systematic effects of exercise on circulating irisin were found in several other studies [10][11][12][13][14][15][16][17] .
Second, the FNDC5 antibody used in the initial study 1 was raised against the C-terminus of the protein (amino acids [aa] 149-178), which is not part of the cleaved irisin peptide (aa 32-143; GenPept accession number NP_715637). Thus, as initially noted by Erickson 18 , the 20 kDa band detected in western blots in that study should not be irisin, but is probably a non-specific cross-reacting protein. Further studies employed western blots with different antibodies against this epitope and found immune-reactive bands in the range of 20-26 kDa in serum or plasma of rats, mice and humans [19][20][21][22] . Again, all these antibodies were generated against the C-terminal FNDC5 segment, which is not part of circulating irisin. An antibody raised against partial irisin (aa 42-112), which should detect irisin, stained a band at 25 kDa as well as bands of higher molecular weight in western blots of the secretome of cultured rat muscle cells and adipocytes 21 . In previous studies, we used an antibody against full-length irisin (aa 32-143) and observed an immune-reactive band at ,13 kDa, the theoretical size of non-glycosylated irisin, in murine serum but not in bovine plasma 23,24 .
The therapeutic potential of irisin to fight obesity and diabetes has aroused extensive interest. Several commercial sources have marketed kits for ELISA, EIA, and RIA to detect and quantify irisin in different biological fluids, under different exercise interventions and/ or in different diseases (reviewed by Sanchis-Gomar et al. 25 , Elsen et al. 26 ). A striking feature of these investigations was the vast variation of reported irisin levels, differing by orders of magnitude even in healthy subjects. This was the case for results obtained with tests from different manufacturers but also with the same test from a single supplier used in different laboratories. More than 80 studies have been published with irisin levels ranging between 0.01 and more than 2,000 ng/mL in human serum or plasma. These commercial kits were usually tested for the ability to detect bacterially expressed irisin (recombinant protein produced in the bacterial cytoplasm, and therefore not glycosylated), and a wide range of sensitivity was reported. For example, a polyclonal antibody used in an ELISA kit (designated here pAb-A) was reported by the supplier to have a detection range of 0.38-205 ng/mL, and a linear range of 4-41 ng/ mL. We believe this is an acceptable validation of the sensitivity of the ELISA kit for assaying purified recombinant irisin, in the absence of other proteins.
However, in addition to recognizing the specific antigen, polyclonal antibodies often cross-react with non-specific proteins. When an ELISA kit, based on a polyclonal antibody against irisin, is used to assay a complex protein sample such as plasma or serum, the signal will include that from any irisin but also that from cross-reacting proteins. Some of these may be present at high levels and can dominate the signal. To control for this, it is necessary to validate the antibody by western blot in the target tissue or fluid. Western blots were shown in the original study 1 , but these showed multiple strong cross-reacting proteins and were based on an inappropriate polyclonal antibody 18 . Only a few of the subsequent studies have presented full-size western blots that reveal cross-reacting proteins in plasma or serum 21,23,24,27 . Many studies using only ELISA have apparently ignored the possibility of cross-reacting proteins contributing to the signal.
In our present study, we examined the specificity and sensitivity of currently available irisin antibodies by comparative western blot analysis. For standards we produced recombinant non-glycosylated irisin (rNG-irisin), which runs on SDS-PAGE as a sharp band at 13 kDa, and recombinant, glycosylated irisin (rG-irisin), expressed in mammalian HEK293 cells and running as a smeared band at 18-25 kDa, with a peak at about 20 kDa. By diluting rNG-irisin into plasma and running western blots we achieved two calibrations: determination of the sensitivity of the assay for irisin, and detection of cross-reacting proteins of various molecular weights. We then extended the assay to survey a range of animal species for the presence of irisin in serum or plasma. In addition, we analyzed samples from a previously published training intervention study 13 with an additional ELISA and western blots to compare irisin levels obtained with different methods. Mass spectrometry was used to search for FNDC5 or irisin signatures in human serum at different sizes after SDS-PAGE. Finally, RNA sequencing was employed to gain insight about the abundance of different transcripts of FNDC5 in human muscle.

Results
Detection of rNG-irisin with pAb-A. Dilution series of rNG-irisin into either phosphate buffered saline (PBS) or bovine plasma were analyzed with anti-irisin pAb-A, raised against full length NG-irisin (Fig. 1a). Bovine plasma was used for the initial test because our previous study had shown no detectable circulating irisin 24 . Two murine sera with unknown irisin levels, human serum samples with irisin levels previously measured with a corresponding ELISA kit (based on pAb-A), and a murine muscle sample were analyzed on the same blot. The antibody reacted with a single band at ,13 kDa in PBS and bovine plasma containing the higher concentrations of added rNG-irisin (Fig. 1a). This band could be completely quenched by preincubation of the primary antibody with 5-fold the amount of rNG-irisin (Fig. 1b). Densitometric analysis of the irisin dilution in bovine plasma revealed linearity in the range from 4 to 0.125 ng irisin/lane (lanes 10-15 in Fig. 1a; R 2 5 0.9947, Fig. 1c). Recovery   rates for the spiked irisin ranged from 75% to 96% in the lanes used for determination of linearity. Because the sample contained 1 mL of plasma we were able to detect irisin at a concentration of 125 ng/mL with this western blot.
The western blot showed several cross-reacting proteins staining much more intensely than even the higher concentrations of added NG-irisin. The band at ,45 kDa is approximately the size for IgG. However, cross-reaction of IgG with the secondary detection system can be excluded because the TrueBlotH system used here is designed to avoid reaction with denatured IgG, and control blots omitting the primary antibody showed no unspecific staining. The band at ,30 kDa is too large to be irisin. The smear from 50-70 kDa and the higher molecular weight bands include albumin and unknown proteins. However, it is clear that these cross-reacting proteins would dominate the signal if this pAb-A is used to assay bovine plasma or human serum.
Notably, irisin was not detected by pAb-A in human serum although levels between 324 and 864 ng/mL were previously reported using the corresponding ELISA kit 13 . This is equivalent to absolute amounts between 0.324 and 0.864 ng/lane on the blot (Fig. 1a), clearly in the linear range of detection by our western blot. A weak band slightly smaller than rNG-irisin was visible in murine serum, but as discussed below is apparently not irisin.
The experiment was repeated with antibodies pAb-B, C, and D ( Supplementary Fig. 1). All antibodies proved capable of detecting rNG-irisin added to PBS or bovine plasma in a similar linear range. However, none of them stained a band of the expected size for glycosylated irisin in murine or human samples. A strong immunereactive band at ,25 kDa was observed only with pAb-C in human but not in murine samples which is the upper size limit for glycosylated irisin. However, this band was not detected by any other irisin antibody, thus it is probably a cross-reaction specific for pAb-C. Analysis of serum samples with pAb-E (raised against aa 149-178 of FNDC5) revealed no specific band in serum but in murine muscle extract it stained a sharp, single band with the size of full length FNDC5 (,24 kDa; Fig. 2a). The strong doublet band at about 25 kDa stained uniquely by pAb-C (Fig. 2b) was tested for deglycosylation. In contrast to rG-irisin, whose size was significantly reduced by PNGase, the ,25 kDa doublet was unaffected (Fig. 2b,c). This band was analyzed by mass spectrometry, described later.
Besides the variability in band patterns observed between the tested antibodies, a high lot-to-lot variability was observed for antibodies pAb-A and C ( Supplementary Fig. 2). Different lots of antibodies pAb-B and D were not compared.
Western blot analysis of irisin in serum and plasma of mammalian species. Sera were analyzed from 3 horses undergoing extreme physical exercise (160 km endurance race) and from 1 horse with established metabolic syndrome. For comparison, samples from cattle, domestic pig, wild boar, donkey, goat, rabbit and mouse were included (Fig. 3). Human samples with irisin levels of 76 ng/mL and 864 ng/ mL, previously measured with ELISA (pAb-A), were from 1 healthy and 1 pre-diabetic individual prior to or 2 hours after a single bout of acute exercise 13 . All samples were analyzed by western blot with antibodies pAb-A and pAb-C (Fig. 3a,b). Recombinant NG-and rGirisin were used as positive controls. No antibody detected proteins at the size of the positive controls, indicating that neither non-glycosylated nor glycosylated irisin circulated in the serum/plasma of any species. However, immune-reactive bands were observed at ,16 kDa in a human sample and at ,25 kDa in both human and goat samples with pAb-C. These bands were consequently further analyzed by HPLC/mass spectrometry.
Separate analysis of 11 samples from 6 horses with pAb-C showed a similar band pattern to that in lanes 3-6 ( Fig. 3b). Band volumes were not related to physical activity or disease and were not correlated to results obtained with pAb-B on the same samples (Supplementary Fig. 3).
Comparison of ELISA data with semi-quantitative western blot analyses. A total of 156 serum samples from 26 individuals from a previously published exercise study 13 were re-analyzed with an additional irisin ELISA based on pAb-C and by semi-quantitative western blot analysis with antibody pAb-C. Irisin levels were previously measured with ELISA based on pAb-A and the data were used for correlation analyses with the results of the present study. Western blot analyses revealed 3 prominent bands at ,16 kDa (larger than rNG-irisin), ,25 kDa, and ,30 kDa in all samples (Fig. 4a). The 16 kDa band was not observed in previous tests with pAb-A (Fig. 1a). Regions of interest were selected and individual band volumes were measured densitometrically (Fig. 4b). There was no significant correlation between normalized volumes of any of the 3 bands or their combined volumes and irisin concentrations measured with the corresponding ELISA kit (pAb-C; r 5 20.109, p 5 0.46; Fig. 4c). Furthermore, irisin levels measured with the 2 different ELISA kits differed by a factor 18 on average (overall means 2,961 ng/mL vs. 164.5 ng/mL) and were not correlated at all (r 5 0.034, p 5 0.67; Fig. 4d). In contrast, the volume of a non-specific band observed at ,50 kDa with pAb-A ( Fig. 4e) was significantly correlated to irisin levels measured with the corresponding ELISA (pAb-A) in an arbitrarily selected subset of samples (n 5 24, r 5 0.730, p , 0.001; Fig. 4f). Thus, using the ELISA based on pAb-C (Supplementary Fig. 4) we were unable to repeat the previous finding of an acute effect of exercise on irisin levels in serum, which used an ELISA based on pAb-A 13 .
Immuno-precipitation and protein identification by mass spectrometry. The immuno-reactive bands at ,25 kDa stained by pAb-C in human ( Supplementary Fig. 1b, Fig. 3b) and goat serum (Fig. 3b) were purified by immuno-precipitation with pAb-C cross-linked to magnetic beads (Fig. 5a,b). An additional band at ,16 kDa observed earlier in human serum (Fig. 3b) was also included in subsequent mass spectrometric analysis. No peptide corresponding to FNDC5 or its irisin-part was identified in any of the precipitated samples in 2 repeats of the experiment. Instead, apolipoprotein A1 was identified as a predominant protein in both human and goat bands. The human band of lower molecular weight included mainly Ig kappa chains and hemoglobin subunits.
We then spiked rNG-and rG-irisin into human sera and analyzed bands matched to the size of the recombinant control peptides by HPLC/mass spectrometry (Fig. 5c,d). Samples of human serum without addition of rNG-or rG-irisin were applied to the same gels. Gel pieces at the positions corresponding to the size of the controls were cut out and analyzed by HPLC/mass spectrometry even though no staining with Coomassie Blue was observed. Unique peptide signatures for FNDC5 or irisin (MLRFIQEVNTTTR or FIQEVNTTTR, Fig. 6a) were detected in samples with added 100 ng or 500 ng rNGirisin in bands at ,13 kDa. The doublet band visible in human serum without addition (lane 4 in Fig. 5c) contained no FNDC5 or irisin. Specific signatures for FNDC5 or irisin were found at ,20 kDa in human serum with 100 ng or 500 ng rG-irisin added. The respective gel piece from a serum sample without added rG-irisin (lane 4 in Fig. 5d) revealed no visible band but contained a single peptide signature unique for FNDC5 or irisin (FIQEVNTTTR) among 13 other proteins. Besides this, the results from mass spectrometry supported the assumption of massive non-specific reaction of proteins with irisin antibody pAb-C.
Expression of FNDC5 mRNA in human skeletal muscle. Re-analysis of samples of m. vastus lateralis from control and pre-diabetic subjects in a training intervention study by RNA-sequencing confirmed a small but statistically significant increase of total FNDC5 mRNA levels after 12 weeks of exercise compared to baseline levels (fold change 5 1.185, p , 0.001). Effects of the health status were not observed in accordance to the previous analysis 13 .
The current NCBI RefSeq model of the human FNDC5 gene predicts 3 transcripts (T1: NM_001171941.2, T2: NM_153756.2, T3: NM_001171940.1). Transcript 1 is proposed to be produced by alternative splicing from an upstream exon 1a, whereas the two other transcripts share exon 1b (Fig. 6a). Transcript 1 would produce a truncated FNDC5 protein from an in-frame AUG codon in exon 3, and the proposed irisin peptide would miss the first 44 amino acids.
However, the Kozak sequence around this AUG is very weak and there are 3 other upstream out-of-frame AUG codons that would make translation of this truncated protein unlikely. Thus, analysis of 154 muscle biopsies in the training intervention study 13 revealed no expression of exon 1a specific for this transcript. In contrast, transcripts 2 and 3 were expressed at high levels as indicated by the data of their shared exon 1b (Fig. 6b). A dissection of the expression to specific transcripts 2 or 3 is not possible due to the structure of the locus (Fig. 6a). All investigated 26 individuals possessed the noncanonical AUA start codon.

Discussion
Almost 3 years after the discovery of irisin 1 the debate over its potential role in human metabolism has not been settled. Only recently, Elsen et al. 28 noted that the role of irisin in humans appears still questionable despite the numerous studies. Spiegelman & Wrann 29 commented in reply that there are more than 45 articles reporting human irisin levels measured with a variety of immunoassays with independently derived antibodies and thus they considered the existence of human irisin in blood a closed issue. However, not one of those studies has addressed the possibility that the immunoassays might be reporting cross-reacting proteins in serum or plasma, and not irisin itself.
Our present study examined 4 antibodies of which 3 were used in corresponding ELISAs. More than 80 studies have been published based on these assays. We showed that all antibodies had prominent cross-reactions with non-irisin proteins in serum or plasma of different species. For one antibody we found a strong correlation between the volume of a prominently stained ,50 kDa band in human serum, which is clearly not irisin, and irisin levels measured with the corresponding ELISA. In contrast, no correlation was found between irisin levels measured with 2 different ELISA kits. Earlier comparisons of different ELISA kits reported only weak correlations and considerable differences in absolute values 12,30 . Our data indicate that all previously published assays based on commercial ELISAs, including the more than 45 articles mentioned by Spiegelman & Wrann 29 , were reporting unknown cross-reacting proteins.  Importantly, our Western blots failed to detect irisin or glycosylated irisin in plasma or serum of human and several animal species, even after extreme exercise in the case of horses. A 13 kDa band in mouse serum, identified previously as potential irisin 23 , was stained by pAb-A, but not by -B, -C or -D. This strongly suggests that it is also a cross-reacting protein specific to pAb-A. The detection limit of the most sensitive western blots was about 100 ng/mL being a potential limitation of this study. However, this corresponds to approximately 10 nM which is below the concentration of recombinant FNDC5 or irisin (20 nM) used in cell culture assays 1,31 . Because hormones are typically present in circulation at pM to nM concentrations, it cannot be ruled out that circulating irisin at less than 10 nM could have activity in vivo.
Our data on FNDC5 expression in skeletal muscle demonstrate that the human gene is exclusively translated from transcripts with a mutant start codon. Considering the translation efficiency of such transcripts -1% compared to transcripts with a canonical start codon 3 -very low amounts of FNDC5 could be expected in skeletal muscle. Hence, it is unlikely that irisin can be cleaved and secreted into circulation at amounts measured with ELISAs previously. This is further supported by the observation that acute exercise did not cause a significant up-regulation of FNDC5 and that the mRNA abundance increased only slightly after 12 weeks of training intervention.
In contrast to most studies, which have neglected the possibility of cross-reaction, a recent study 22 employed western blots of human serum as the first step. These blots showed several strong cross- reacting bands of higher molecular weight, but the study focused on bands of 32 and 24 kDa, interpreting them as glycosylated and deglycosylated irisin. As shown in our present report the molecular weights of purified rG-and rNG-irisin are actually 20 and 13 kDa, so the bands identified in that study 22 are too large. Furthermore, they used the same antibody as Boström et al. 1 , designated pAb-E here, which was raised against a C-terminal sequence that is not included in irisin. Finally, they analyzed the 32 and 24 kDa bands by mass spectrometry and reported a single tryptic peptide corresponding to the irisin sequence. We did not find any FNDC5 or irisin signatures in our immune-precipitated bands identified by western blots of human serum. However, we were able to detect irisin spiked into human serum at the predicted size for glycosylated and nonglycosylated irisin.
Mass spectrometry analysis of the ,20 kDa zone cut from SDS-PAGE of human serum detected a peptide corresponding to FNDC5 or irisin. This is the same peptide identified by Lee et al 22 , although they found it in ,32 and ,24 kDa bands stained with a pAb against C-terminal FNDC5. Consequently, our result is the first mass spectrometry identification of an irisin peptide at the correct size, and might be considered as supporting the existence of irisin in human serum. However, none of the tested irisin antibodies stained a band at this size in serum. This underlines that glycosylated irisin has not been detected by western blot before and that none of the ELISA kits used in previous studies have measured irisin levels in serum or plasma of any species.
The birth of the new, promising myokine irisin 1 has been complicated from the beginning by multiple contradictions and obscurities in the interpretation of western blot data 18 . Numerous subsequent studies relied on ELISAs with commercial antibodies that were not sufficiently validated in biological fluids. This resulted in highly contradictory data concerning the existence of irisin and its role in humans and other species. Our study targeted the basic question behind the controversy -the physical existence of the proposed cleavage product of FNDC5 in circulation. The key to this was to use western blots to visualize and avoid the false signal from crossreacting proteins of the polyclonal antibodies, and to use recombinant, non-glycosylated and glycosylated irisin as positive controls. In addition to calling into question all previous studies using commercial ELISAs, we found no evidence for circulating irisin in human or several animal species when examined by western blot with 4 different antibodies and a sensitive detection system. Although we report the first peptide signature size-matched to rG-irisin by HPLC/mass spectrometry in human serum, the apparently low concentrationbelow the detection limit of the tested antibodies -makes a physiological role for irisin very unlikely. Atherton & Philips 32 raised the question whether irisin is a ''Greek goddess or a Greek myth''. Our results provide experimental evidence for irisin being a myth.

Methods
Ethical approval. The human exercise study named MyoGlu adhered to the Declaration of Helsinki and was approved by the National Regional Committee for Medical and Health Research Ethics North, Tromsø, Oslo, Norway. The study was registered with the US National Library of Medicine Clinical Trials registry (NCT01803568). Written informed consent was obtained from all participants prior to any study-related procedure. Equine blood samples were taken in accordance with the local animal ethics committee (Etat de Vaud, Service Vétérinaire, Switzerland). Strength and endurance training intervention. Healthy, sedentary men (40-65 years) were recruited in 2 groups; controls with normal weight (23.5 6 2.0 kg/m 2 ) and normal fasting and 2 h serum oral glucose tolerance test (OGTT) levels (n 5 13) or overweight (29.0 6 2.4 kg/m 2 ) with abnormal glucose metabolism (pre-diabetes group, n 5 13). Abnormal glucose metabolism was defined as fasting glucose $ 5.6 mmol/L and/or impaired glucose tolerance (2 hours serum OGTT $ 7.8 mmol/ L). The participants underwent 2 endurance bicycle sessions (60 min) and 2 wholebody strength-training sessions (60 min) per week for 12 weeks. A 45 min bicycle session at 70% of VO 2 max was performed before and after the 12 week training period as an acute work challenge 13 .
Blood and tissue sampling. Blood and muscle samples were taken before, directly after, and 2 h after the 70% of VO 2 max bicycle test, before as well as after 12 weeks of training. Blood samples were taken by standard antecubital venous puncture. Biopsies from m. vastus lateralis were immediately rinsed in phosphate buffered saline (PBS), dissected free of blood, before they were transferred to RNA-later (Qiagen, Limburg, Netherlands; overnight at 4uC) and finally transferred to 280uC. Serum was stored at 280uC until analysis.
Animal serum and plasma samples. Samples for species comparison were obtained from commercial suppliers (donkey, goat, rabbit), from previous studies in swine and wild boar 33 , and from our own investigations in cattle 24   The open box marks the truncated irisin peptide in P1 theoretically resulting from transcript T1. The size of irisin and FNDC5 protein variants is given. The peptide signature identified by mass spectrometry is marked in red. SP: signal peptide. (b) Example for detection of FNDC5 transcripts by RNA-sequencing of skeletal muscle biopsies. Exon 1a is specific for transcript T1 whereas exon 1b is part of transcripts T2 and T3. The panels represent results for one individual before (A1-A3) and after 12 weeks of training intervention (B1-B3). A1 and B1 were measured before, A2 and B2 immediately after, and A3 and B3 2 hours after acute exercise 13 . www.nature.com/scientificreports SCIENTIFIC REPORTS | 5 : 8889 | DOI: 10.1038/srep08889 France). One horse with confirmed metabolic syndrome was sampled at the Swiss Institute of Equine Medicine (ISME), Vetsuisse Faculty, University of Berne.
Expression and purification of recombinant, non-glycosylated and glycosylated irisin. For convenient his-tag removal, the bacterial irisin expression construct 34 was modified by inserting the 3C protease cleavage sequence (LEVLFQGP) in the Nde I site. This modification dramatically reduced protein solubility when it was expressed in E. coli BL21 (DE3) at 37uC, so the his-3C-irisin construct was expressed in E. coli C41 (DE3) 35 at room temperature. Expressed proteins were purified with a cobalt column (TALON; Clontech, Mountain View, USA) using standard protocols. Purified proteins eluted with imidazole from the column were dialyzed against phosphate buffered saline (PBS, pH 7.2) in the presence of his-tagged 3C protease at 4uC. After dialysis, samples were run through the cobalt column again to remove uncleaved his-tagged irisin, cleaved his-tagged portions and his-tagged 3C protease. Non his-tagged irisin was recovered in the flow through fraction from the cobalt column. The amino acid sequence of rNG-irisin (no his-tag) is gphmSPSAP-VNVTVRHLKANSAVVSWDVLEDEVVIGFAISQQKKDVRMLRFIQEVNTTT-RSCALWDLEEDTEYIVHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDE-VTMKE (the sequence in lowercase is derived from the vector). Recombinant Girisin was produced in mammalian cells 34 . The irisin expression construct (irisin/ pHLSec2) was transfected into HEK293 cells and the conditioned medium was collected after 6-7 days of transfection. Secreted protein was purified with the cobalt column and the C-terminal his-tag was not removed. The amino acid sequence of rGirisin is egsADSPSAPVNVTVRHLKANSAVVSWDVLEDEVVIGFAISQQKKDV-RMLRFIQEVNTTTRSCALWDLEEDTEYIVHVQAISIQGQSPASEPVLFKTPR-EAEKMASKNKDEVTMKEefhhhhhhh. Glycosylation was verified by a molecular weight shift in SDS-PAGE after PNGase F (NEB, Ipswich, USA) treatment. The concentrations of purified proteins were estimated from the absorbance at 280 nm using the extinction coefficients of the proteins determined by the Protean computer program (DNAstar, Madison, USA).
In one experiment synthetic irisin (Catalog # 067-16; Phoenix Europe, Karlsruhe, Germany) was used as an additional control.
Antibodies. The following antibodies were used in this study: pAb-A, rabbit polyclonal antibody (pAb) to irisin (Phoenix Europe, Karlsruhe, Germany); pAb-B, rabbit pAb to irisin (Cayman Chemicals, Ann Arbor, USA); pAb-C, rabbit pAb to irisin (AdipoGen, Liestal, Switzerland); pAb-D, rabbit pAb to irisin (Phoenix Europe); and pAb-E, rabbit pAb to FNDC5 (C-terminal; BioCat, Heidelberg, Germany). Antibodies pAb-A and C were raised against full length rNG-irisin and pAb-B and D against the full-length synthetic irisin peptide. Antibody pAb-E was generated against the C-terminus of FNDC5, which is not part of the irisin peptide. Details on the antibodies are given in Table 1.
Western immunoblotting. For dilution series, rNG-irisin was diluted into either PBS or bovine plasma. Irisin stock solution (1 mg/mL in PBS) was first diluted 15100 in bovine plasma resulting in minimal dilution of plasma proteins. This recombinant NG-irisin solution was further diluted in bovine plasma to final concentrations of 10, 2, 1, 0.5, 0.25, 0.125, 0.0625, and 0.03125 ng/mL. Two microliters of each sample were then mixed 152 with loading buffer (23 Laemmli sample buffer with 10% bmercaptoethanol), denatured at 95uC for 5 min and separated on Criterion TGX 12% polyacrylamide gels (BioRad). Absolute amounts of rNG-irisin analyzed per lane were consequently between 20 ng and 0.0625 ng. Each 1 mL of native murine and human serum samples and 20 mg murine muscle protein extract were treated as described above and electrophoresed on the same gel.
For species comparison, 1 mL of serum or plasma from the following species was used: horse, cattle (bull, cow, calf), pig (domestic and wild), donkey, goat, rabbit, mouse, and human. Samples were analyzed under the same conditions as described above. Recombinant NG-irisin (2 mL from 1 ng/mL stock solution) and rG-irisin (1 mL from 10 ng/mL stock solution) were used as positive controls.
Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Trans-Blot Turbo transfer pack, Bio-Rad, Munich, Germany) using a semi-dry blotter (Trans-Blot, Bio-Rad). Equal loading of the gels and proper transfer of the proteins to the membranes were verified by Coomassie Blue staining according to standard procedures. Membranes were blocked for 1 h in either 13 Roti-Block (Roth), for detection of irisin (pAb-A, B, C and D), or in 5% non-fat dry milk in Trisbuffered saline (TBS) for detection of FNDC5 (pAb-E). Membranes were incubated with primary antibodies (pAb-A: diluted 153,000, pAb-B: 15500, pAb-C: 152,000, and pAb-E 151,000) overnight at 4uC.
Analysis of serum irisin. Concentrations of irisin were measured in duplicates in human serum using enzyme-linked immuno-sorbent assays (ELISA [based on pAb-C]; Table 1) according to the manufacturer's protocol. Optical density was determined using a microplate reader (Termo Scientific Multiscan EX, Vantaa, Finland) set to 450 nm. Data from a previous measurement of irisin in plasma samples of the same individuals 13 with a different ELISA (based on pAb-A; Table 1) were used for comparison. Letters assigned to the ELISAs correspond to the letters designated to the antibodies.
Immuno-precipitation and SDS-PAGE of serum samples with added irisin. MagnaBind magnetic beads (Pierce, Thermo Fisher Scientific, Bonn, Germany), precoated with anti-rabbit IgG antibody, were incubated with anti-irisin antibody (Adipogen) for 60 min under permanent agitation (Dynabeads MX Mixer, Life Technologies, Darmstadt, Germany). Separation steps were performed using InviMag (STRATEC, Berlin, Germany) magnetic separator. Binding of antibodies to the beads was made permanently according to standard protocols by using crosslinking buffer (25 mM DMP) at room temperature for 45 min. To stop the reaction, blocking buffer (0.1 M Ethanolamine in PBS, pH 8.2) was used. Unbound antibody was eluted from the beads with elution buffer (1 M glycine-HCL, pH 3). Serum (500 ml) was denatured for 5 min at 95uC and added to the antibody coated beads. Incubation was performed overnight at 4uC under permanent agitation. After several washes, proteins were eluted from beads with elution buffer (1 M glycine-HCL, pH 3). To optimize the protocol for subsequent mass spectrometry, the elution buffer was changed (8 M urea, 20 mM Tris pH 7.5, 100 mM NaCl) according to standard protocols (abcam, Cambridge, U.K.) in a repeated trial.
Eluted proteins were separated on a 15% polyacrylamide gel and stained with QuickCoomassie (Serva, Heidelberg, Germany) according to manufacturer's instructions. Bands of interest were cut out of the gel and submitted to analysis by mass spectrometry. Furthermore, human serum samples were albumin-depleted with Aurum Affi-Gel Blue mini columns (Bio-Rad) as described elsewhere 24 . One hundred or 500 ng of either rNG-or rG-irisin were then added to the samples. The samples (20 mg total protein) were separated by SDS-PAGE (15%) and stained with QuickCoomassie (Serva). Serum samples without addition of rNG-/rG-irisin were also analyzed. Recombinant NG-/rG-irisn (500 ng) diluted in PBS were used for size comparison. Regions of interest were cut out of the gel and analyzed by mass spectrometry.
Protein identification by mass spectrometry. The Coomassie stained gel bands were in-gel digested using trypsin. The generated peptides were purified using m-C18 ZipTips (Millipore, Oslo, Norway), dried in a SpeedVac, dissolved in 10 mL 1% formic acid, 2.5% acetonitrile in water. Half of the volume was injected into a nano-UHPLC system (Ultimate 3000 RSLC, Dionex, Sunnyvale, CA, USA) coupled to an ESI-ion trap/Orbitrap (LTQ Orbitrap XL, Thermo Scientific, Bremen, Germany) mass spectrometer. For peptide separation, an Acclaim PepMap 100 column (50 cm 3 75 mm) packed with 100 Å C18 3 mm particles (Dionex; Nerliens Meszansky, Oslo, Norway) was used with a flow rate of 300 nL/min and a solvent gradient of 3% B to 35% B for 45 min. Solvent A was 0.1% formic acid and solvent B was 0.1% formic acid/90% acetonitrile. Survey full scan MS spectra (from m/z 300 to 2000) were acquired in the Orbitrap with the resolution R 5 60,000 at m/z 400 after accumulation to a target of 1,000,000 charges in the LTQ. The method allowed sequential isolation of up to the 7 most intense ions for fragmentation on the linear ion trap using collision induced dissociation (CID) at a target value of 10,000 charges. Target ions already selected for MS/MS were dynamically excluded for 60 sec. Data were acquired using Xcalibur v2.5.5 (Thermo Scientific) and raw files were processed to generate peak list in Mascot generic format (*.mgf) using ProteoWizard release version 3.0.331 36 . Database searches were performed using Mascot in-house version 2.4. to search the SwissProt database assuming the digestion enzyme trypsin, at maximum one missed cleavage, fragment ion mass tolerance of 0.60 Da, parent ion tolerance of 10 ppm and oxidation of methionines, and acetylation of the protein N-terminus as variable modifications. Scaffold (version Scaffold_4.3.4, Proteome Software, Portland, USA) was used to validate MS/MS based peptide and protein identifications. Peptide identifications were accepted if they could be established at greater than 95% probability by the Scaffold Local FDR algorithm. Protein identifications were accepted when they could be established at greater than 99% probability and contained at least 2 identified peptides.
High throughput mRNA sequencing and differential expression analysis. Skeletal muscle biopsies sampled before the start of acute exercise at baseline and after 12 weeks of training were deep-sequenced in 6 batches using the Illumina To avoid low quality data negatively influencing downstream analysis, reads were trimmed on the 39-end and only the first 51 bp from the 59-end of each read were kept for further analysis. The mean library size was ,44 million unstranded 51 bp single-ended reads with no difference between groups or time points. Base composition in bases 1-12 showed patterns typical for RNA-seq and bases 13-51 were evenly distributed. All base positions were of high quality (Phred score .30). Alignment of sequenced cDNA reads to the RefSeqGene database as transcriptome reference and quantitative analysis of the transcripts was done as described by Li et al. (2014) 37 .
Statistical analysis. ELISA and western blot data were analyzed with ANOVA using PROC MIXED (SAS Institute, Cary, USA) with the following factors included in the model: fixed factor group and repeated factor treatment (with unstructured covariance structure) and corresponding interaction, plate and blot were included as random effect, respectively. Differences between means were considered significant if p , 0.05. Pearson correlation coefficients were calculated among quantitative data from ELISAs and western blots.