Table 1 Total sonication energy input versus cell suspension volume for maximal CFPS productivity

From: High-throughput preparation methods of crude extract for robust cell-free protein synthesis

Strain Cell suspension volume (μL) Sonication energy (J)  
Predicted energy input for >95% productivity Predicted energy input for 100% productivity Observed energy input for 100% productivity Difference (%)a
BL21 Star™ (DE3) 100 24 ± 11 37 24 54.2
300 143 ± 39 150 140 7.1
500 299 ± 71 263 309 15.0
750 426 ± 72 404 398 1.5
1000 639 ± 206 545 532 2.4
1500 798 ± 105 826 822 0.5
K12 MG1655 C495 100 23 ± 6 −10 26 138.0
300 146 ± 32 145 138 5.0
500 290 ± 38 300 285 5.1
700 417 ± 143 454 414 9.7
1000 613 ± 151 686 705 3.0
1500 940 ± 230 1073 1080 3.0
  1. (a)Energy differences between predicted and observed 100% productivity.