Figure 4 | Scientific Reports

Figure 4

From: High-throughput preparation methods of crude extract for robust cell-free protein synthesis

Figure 4

Mapping CFPS yields across the landscape of total sonication energy input and cell suspension volume demonstrates the ability to generate highly active extracts.

CFPS activity was mapped for two E. coli strains, showing a linear trend for the highest CFPS activity. The color code represents relative CFPS activities (100% (red) to 0% (purple)) for each designated energy and volume. Linear trends for total energy inputs covering the highest CFPS activity are computed as follows: Equation for BL21 Star™ (DE3): [Energy] = [Volume(μL)-33.6]·1.8−1, Equation for C495: [Energy] = [Volume(μL) − 112.7]·1.3−1.

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