Figure 8 : MANF suppresses the proliferation of inflammatory FLS.

From: Mesencephalic Astrocyte-Derived Neurotrophic Factor Is Involved in Inflammation by Negatively Regulating the NF-κB Pathway

Figure 8

(a) MANF over-expression represses FLS proliferation. FLS were transfected with MANF-FLAG and treated with TM (2.5 μg/ml) for 24 hrs. FLS viability was determined using an MTT assay. MANF expression was detected by Western blotting. The data are the means ± SD of at least three independent experiments. * P = 0.03, compared with vector control. (b-c) Recombination MANF protein inhibits FLS proliferation. The FLS isolated from AA rats were cultured in DMEM medium containing 2.5 μg/ml TM and different concentrations of MANF protein (as indicated) for 24 hrs (b) and 48 hrs (c). BSA and DXM were used as negative control and positive control, respectively. FLS viability was determined using an MTT assay. The data are the means ± SD of at least three independent experiments. * P = 0.017, ** P = 0.008, *** P = 0.001, # P = 0.021, ## P = 0.003, ### P = 0.007, compared with the BSA control. (d) MANF-siRNA promotes FLS proliferation. The normal and inflammatory FLS were transfected with MANF-siRNA and control siRNA for 24 hrs, then treated with TM (2.5 μg/ml) for 24 hrs. FLS viability was detected using an MTT assay. The data are the means ± SD of at least three independent experiments. * P = 0.041, # P = 0.011, compared with the siRNA controls.