Table 3 Amplification of MP, hMPV and hBoV genes

From: Bronchiolitis Associated With Mycoplasma Pneumoniae in Infants in Suzhou China Between 2010 and 2012

Gene   Sequence Size (bp)
MP Forward 5′-CCA ACCAAA CAA CAA CGT TCA-3′ 76
  Reverse 5′-ACC TTG ACTGGA GGC CGT TA-3′  
  Probe 5′-FAM-TCA ACT CGA ATA 'ACG GTG ACTTCT TAC CAC TG-3′-TAMRA  
hMPV Forward 5′-AACCGTGTACTAAGTGATGCACTC-3′ 213
  Reverse 5′-CATTGTTTGACCGGCCCCATAA-3′  
hBoV Forward 5′-TGACATTCAACTACCAACAACCTG-3′ 92
  Reverse 5′-CAGATCCTTTTCCTCCTCCAATAC-3′  
  Probe 5′-FAM-AGCACCACAAAACACCTCAGGGG-3′-TAMRA  
  1. MP, Mycoplasma pneumonia; hMPV, human metapneumovirus; hBoV, human bocavirus; FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.