Table 1: Application of MuDT technique to detection of sexually transmitted infections in clinical samples

From: Single-channel multiplexing without melting curve analysis in real-time PCR

 Melting curve analysisMuDT (Ct value)
 Ch 1Ch 2Ch 3Ch 4Ch 1Ch 2Ch 3Ch 4
Sample No.UUUPMGMHNGCTICTVUUUPMGMHNGCTICTV
1++ND+ND++ND38.1234.37ND38.33ND29.0932.68ND
2+NDNDND+++ND39.53NDNDND29.3336.6230.88ND
3ND+ND+ND++NDND38.41ND27.02ND27.2531.50ND
4++NDND+ND+ND34.8728.24NDND28.88ND32.12ND
5++NDNDNDND+ND40.5635.19NDNDNDND32.29ND
6++ND++++ND38.2535.53ND25.7528.1732.2331.83ND
7NDND+NDNDND+NDNDND44.04NDNDND31.65ND
8++ND+ND++ND34.3931.87ND28.86ND33.7031.80ND
9NDNDNDND+++NDNDNDNDND28.2130.0533.23ND
10++ND+ND++ND34.8431.91ND28.17ND33.0932.18ND
11+NDNDND+++ND37.09NDNDND30.5231.4633.79ND
12ND+ND++++NDND44.84ND31.3325.4527.4631.76ND
13+NDNDNDND++ND44.00NDNDNDND32.1231.79ND
14ND+++ND++NDND37.7133.5725.45ND28.5834.14ND
15ND+NDND+++NDND30.47NDND35.2926.2333.17ND
16++ND+NDND+ND41.5540.66ND32.28NDND30.87ND
17+ND++ND+++31.63ND33.6824.96ND26.6331.6844.73
18++++ND++ND40.0648.4138.6631.11ND26.4431.85ND
19+NDNDND+++ND41.39NDNDND31.1238.3731.92ND
20+NDNDNDND++ND37.89NDNDNDND29.7631.96ND
  1. Ch 1: Channel 1 (FAM);
  2. Ch 2: Channel 2 (HEX);
  3. Ch 3: Channel 3 (Cal Fluor Red 610);
  4. Ch 4: Channel 4 (Quasar 670);
  5. UU: Ureaplasma urealyticum (low Tm);
  6. UP: Ureaplasma parvum (high Tm);
  7. MG: Mycoplasma genitalium (low Tm);
  8. MH: Mycoplasma hominis (high Tm);
  9. NG: Neisseria gonorrhoeae (low Tm);
  10. CT: Chlamydia trachomatis (high Tm);
  11. IC: Internal control (low Tm);
  12. TV: Trichomonas vaginalis (high Tm);
  13. +: positive;
  14. ND: not detected.