Figure 2 : Identification of the high Tm target by detection temperatures.

From: Single-channel multiplexing without melting curve analysis in real-time PCR

Figure 2

(a) A Multiple Detection Temperature (MuDT) reaction was assembled for the screening of Chlamydia trachomatis (CT) (high Tm), and the fluorescence signal was detected at three different detection temperatures (60°C, 72°C, and 95°C) at each PCR cycle. The first three graphs represent the amplification plots for the target, and the last graph contains the composite melting curves of the targets and three detection temperatures shown as dotted lines. (b and c) Procedures are the same as in (a), except that Neisseria gonorrhoeae (NG) (low Tm) alone (b) or both targets (c) were screened.