The fluorescence microscopy and HRTEM images demonstrating the defective outer membrane/capsid in microbes caused due to Ag+ + CO32− treatment.
Line (a): Fluorescence microscopy images of E. coli: ([i] Input bacteria, [ii] 50 ppb Ag+-treated bacteria and [iii] 50 ppb Ag+ + 20 ppm CO32−-treated bacteria after staining with syto9 and propidium iodide. Line (b): Similar fluorescence microscopy images of S. aureus: [iv] Input bacteria, [v] 50 ppb Ag+-treated bacteria and [vi] 50 ppb Ag+ +20 ppm CO32−-treated bacteria. Line (c) TEM images of MS2 bacteriophage: [vii] Input viruses, [viii] 50 ppb Ag+-treated viruses and [ix] 50 ppb Ag+ + 20 ppm CO32−-treated viruses after staining with 0.2% uranyl acetate. Images (vii) and (viii) appear the same as they are not damaged, while image (ix) appears dark as damage in the capsid allowed the stain to pass through it.