Figure 1 : DHM inhibits cell proliferation and promotes cell apoptosis.

From: A reduction in reactive oxygen species contributes to dihydromyricetin-induced apoptosis in human hepatocellular carcinoma cells

Figure 1

(A): The chemical structure of DHM. (B): DHM induces apoptosis in HepG2 cells at 24 h (a: control, b: 10 μM, c: 50 μM, d: 100 μM), which was visualized by microscopy (×100). (C): Cell growth, as detected by the MTT assay, was inhibited in a concentration-dependent manner at 24 h after DHM treatment. (D): DHM-induced cell apoptosis was analyzed by flow cytometry (24 h). (E): DHM-induced cell apoptosis (100 μM, DNA fragmentation) was visualized by fluorescence microscopy (24 h, TUNEL staining, original magnification: ×100). (F): Number of apoptotic cells. At least six 12 mm2 fields of view were counted in each culture dish. (G): DHM did not inhibit cell proliferation in normal hepatic HL7702 cells. Cell growth was detected by the MTT assay. H: HL7702 cells were treated with 100 μM DHM for 24 h, and cell apoptosis was analyzed using flow cytometry (a: Control, b: 100 μM DHM). Values are the means ± SD for at least three independent experiments performed in triplicate.