Table 4 Primers used for frequency examination and allele-specific PCR

From: Novel mutations and mutation combinations of ryanodine receptor in a chlorantraniliprole resistant population of Plutella xylostella (L.)

Sequencing primer
No. name Primer sequence Length of products (bp)
1 F-3679 GAT ACG AGC CAT TCT GTG T 530 (containing mutation E1338D)
  R-4208 TGC CAT TCT GTT GAC CTG  
2 F-13512 CCC ACG GAA GAG GAG AAA 791 (containing mutation Q4594L and I4790M)
  R-14302 TCC AGT TTA CGG GCT ATC T  
3 F-14596 ACA ACT CGT TCC TAT ACT CTC 152 (containing mutation G4946E)
  R-14747 TGT TTC CCG TTA TGC GTG AC  
Allele-specific primer    
4 F-3979-S ATG AAG GAG GCA GCT CAA 116 (for E1338D genotyping)
  F-3979-R ATG AAG GAG GCA GCG CAT  
  R-4361 TCC TCT TGA CCA TCA TCA TAG T  
5 F-13620-S GGA AGA TAA TGG ACA AGT TCA 168 (for Q4594L genotyping)
  F-13620-R GGA AGA TAA TGG ACA AGT TCT  
  R-13787 GCT TGC TTC TTA GCT TGT TC  
6 F-14677-S TGT TGG ACG TGG CTG TAG G 71 (for G4946E genotyping)
  F-14677-R TGT TGG ACG TGG CTG TAG A  
  R-14747 TGT TTC CCG TTA TGC GTG AC  
  1. The differences of susceptible and resistant allele specific nucleotides were underlined and the mismatched nucleotides were marked in bold.