Figure 4 : Visualization of sequencing read depth in SeqMonk for three amplicons previously used for comparing enzymes.

From: Long-range PCR in next-generation sequencing: comparison of six enzymes and evaluation on the MiSeq sequencer

Figure 4

Left column shows amplicons BRCA1.1, BRCA1.6 and BRCA2.8 and their boundaries. Right column shows junction regions. Arrows indicate boundaries. Coverage is log2-transformed. The coverage plot demonstrates that significant variations of read depth may still exist within and between amplicons from long-range PCR. Loss of coverage may occur at junction of two amplicons (B), but can be recovered by larger overlapping of two amplicons (D, F).