NAC suppressed the blue LED light-induced damage and inhibited NF-κB activation.
(A–C) Evaluation of the cell viability by the CCK-8assay and the rate of cell death by Hoechst and PI staining. The rate of cell death indicates by the ratio of PI (red) stained cells per Hoechst (blue) stained cells. NAC at 1 mM significantly improved the cell viability reduced by blue LED light. (D) NAC reduced the ROS level elevated by blue LED light. (E–H) The effect of NAC against blue LED light-induced changes in protein expression was assessed by Western blots. NAC suppressed the blue LED light-induced increase in activated NF-κB levels, but did not suppress activated p38. NAC did not alter the reduced ERK level. Data are expressed as mean ± SEM (n = 5 or 6). ** indicates p < 0.01 vs. vehicle; ## indicates p < 0.01 vs. control (one-way ANOVA followed by Tukey's test). The scale bar represents 50 μm. The cropped blots are used in this Figure and the full-length blots are presented in Supplementary Figure S8.