The aggregation of S-opsin induced by blue LED light exposure.
(A) Representative immunostaining images of S-opsin after LED light exposure for 24 h. Blue and white LED light-induced the perinuclear aggregation of S-opsin compared to control and green LED light. n = 4. (B) Representative immunostaining images of S-opsin shows that the S-opsin aggregated cells after blue LED light exposure for 3 or 6 h (arrowhead). (C) Quantitative analysis of immunostaining images. The ratio of the S-opsin aggregated cells was increased by blue LED light exposure for 3 or 6 h. Data are expressed as mean ± SEM (n = 3 or 4). # indicates p < 0.05 vs. control (one-way ANOVA followed by Dunnett's test). The scale bars represent 5 μm (A), 50 μm and 10 μm (B).