Figure 4 : Effects of 6-month oral administration of Arg, LKM512, or Arg & LKM512 mix in aging ICR mice.

From: Upregulation of colonic luminal polyamines produced by intestinal microbiota delays senescence in mice

Figure 4

The test sample was administered 3 times a week, starting when the mice were 12 months old. Data were obtained from 7 mice in the Arg and LKM512 groups, 6 mice in the Arg & LKM512 mix group (a mouse died in the study period), and 4 mice in the control group (3 mice died in the study period). (a) Serum inflammatory cytokine concentrations *p < 0.05, **p < 0.01. (b) SMP-30 content in liver tissue (right: western blot, left: immunohistochemical staining). (c) A dendrogram of fecal microbiota of mice after a 6-month treatment based on the T-RFLP pattern. (d) Bacterial PUT synthesis pathway from an Arg precursor. Pathway 1: decarboxylation of ornithine generated from Arg hydrolysis. Pathway 2: direct conversion of agmatine generated from Arg decarboxylation, catalyzed by agmatine ureohydrolase. Pathway 3: indirect conversion of agmatine generated from Arg decarboxylation via N-carbamoylputrescine, catalyzed by agmatine deiminase/iminohydrolase and N-carbamoylputrescine amidohydrolase (e) Comparison of the proportion of PUT synthesis-related genes detected from both the Arg & LKM512 mix group and the control group by fecal metagenome analysis.