Figure 1 : Screening of putrescine-upregulating substances by culture methods.

From: Upregulation of colonic luminal polyamines produced by intestinal microbiota delays senescence in mice

Figure 1

(a) PA production of a single culture of E. coli MG1655, Blautia producta JCM 1471T, or Bacteroides thetaiotaomicron JCM 5827T with candidate substances (GABA, Arg, Lys, Pro, fumaric acid, Asp, and Ser). Candidates that increased PA concentration more than 2-fold were used in experiments that required fecal culture. (b) PUT concentration stimulated by candidate substances with human fecal culture. Data are expressed as relative ratio that calculate PUT concentration from 0 h to 1 h. Results of each fecal specimen are shown in Supplementary Fig. S1A. Data are represented as mean ± SD **p < 0.01, ***p < 0.001. (c) Dose-dependent effect of Arg on PUT production with human fecal culture.