Intercellular ligand-receptor recognitions are crucial natural interactions that initiate a number of biological and pathological events. We present here the simple construction of a unique class of biomimetic interfaces based on a graphene-mediated self-assembly of glycosyl anthraquinones to a screen-printed electrode for the detection of transmembrane glycoprotein receptors expressed on a hepatoma cell line. We show that an electroactive interface confined with densely clustered galactosyl ligands is able to ingeniously recognize the asialoglycoprotein receptors on live Hep-G2 cells employing simple electrochemical techniques. The only facility used is a personal laptop in connection with a cheap and portable electrochemical workstation.
A multitude of life events are modulated by intercellular ligand-receptor recognitions. And it is believed that the interactions between glycans that massively distribute in all living organisms and their specific transmembrane receptors initiate myriad physiological and pathological events such as cell-cell adhesion, cellular differentiation, cell growth and cytokinesis, bacterial infection, viral invasion and cancer metastasis1,2,3,4,5,6,7,8. Consequently, the elucidation of these interactions may contribute to decipherment of the “Glycomics” and facilitate early-state disease diagnosis and sugar-based drug and vaccine development9,10,11,12.
However, the measurement of sugar-protein interactions has been a difficult task because of the low binding affinity between a glycan and its cognate protein. To address this issue, the so-called glycoarray technique has been developed, which is based on the immobilization of neo-glycoconjugates onto a solid surface for detecting fluorescently tagged analytes13,14,15,16,17. Indeed, this elegant method has been of particular interest due to the following merits: (i) a very small amount of sugars are required; (ii) confining sugars on surfaces in a densely clustered manner gives rise to a much enhanced binding avidity with receptors, resulting in signal amplification.
Despite the contribution of this technology towards the progress of the glycomics, however, some flaws remain that hamper its wide-spread utilization. For instance, immobilization of sugar ligands onto solid surfaces requires substantial effort and the biosepcificity of chemically labelled analytes might be compromised. In addition, costly facilities are used for detection, making such a means relatively impractical for the majority of research groups. We present here the unique construction of a simple yet highly biospecific interfacial system based on a one-step graphene-mediated self-assembly of anthraquinonyl glycosides to a screen-printed electrode (SPE) for sensitive detection of intercellular sugar ligand-receptor interactions. We show that the designed system has the ability to exquisitely capture glycoprotein receptors exclusively expressed on live cancer cells employing facile electrochemical methods and economic facilities.
The electroactive glycosyl anthraquinones (GAs) were prepared by a click reaction18,19 of azido glucoside (a) and galactoside (b) with, respectively, a bis-(c) and a mono-(d) O-propargyl anthraquinone20. Triazolyl glycosyl acetates 1′–4′ were afforded with reasonable yields and a subsequent deacetylation led to the desired dimeric and monomeric GAs 1–4 (Fig. 1 and Scheme S1).
Fabrication of the biomimetic interface
Graphene oxide (GO), a low-cost, scalable (vs. other carbonaceous materials such as fullerene and carbon nanotube) yet powerful carbon flake that has fulfilled the fabrication of many ingenious biosensors and devices21,22,23,24,25,26,27,28, was used to facilitate the fixation of the GAs onto SPE. Self-assembly of GAs 1–4 to GO prepared from graphite powder according to the modified Hummers method29 was first realized via π-stacking (Fig. 1a and Fig. 1b). Atom force microscope used to identify the formation of the GO-GAs showed that the height of bare GO is ~1.0 nm (Fig. S1a) which agrees with that of exfoliated GO sheets reported previously30. In contrast, the height of all GO-GAs grew to ~1.7 nm, indicating the adsorption of the GAs onto GO surface (Fig. 1c to Fig. 1f). Raman and Fourier Transformation Infrared (FTIR) spectroscopic analyses further confirmed the formation of the π-stacked composites. All Raman spectra displayed the characteristic D band and G band of GO at 1354 cm−1 and 1604 cm−1, respectively (Fig. S2). The increased ID/IG ratio of GO-GAs (Fig. S2a2-Fig. S2a5) compared to that of GO (Fig. S2a1) signifies an increased disorder of carbon sp2-hybridization due to the presence of the triazolyl anthraquinone of the former. Adsorption peaks assignable to the methylene and triazole groups of GAs were observed in the FTIR spectra of the GO-GAs (Fig. S2b2-Fig. S2b5).
Production of the SPEs that consist of a graphite-based working electrode was actualized according to our previously described method30. These low-cost electrodes can be produced massively, representing an ideal class of electrochemical micro-device. The interfacial systems were then finalized by simply spotting the GO-GAs (3 μL) to the working electrode of SPE. Cyclic voltammetry (CV) was primarily used to depict the electrochemical processes of the interfacial GO-GAs (Fig. 2) in a phosphate buffered saline (PBS) buffer; an electrode confined with GO-1,8-dihydroxyanthraquinone (AQ-OH) was used as control (Fig. S3a). By increasing the scan rates from 0.02 to 0.12 V/s, the CVs of all redox couples showed a pair of symmetric peaks with a gradually increased peak current. Plotting of the scan rates as a function of the corresponding anodic and cathodic peak currents resulted in linear correlations (insets of Fig. 2a–d and Fig. S3b), which means that the oxidation and reduction processes of the electroactive species are surface-controlled. This observation suggests that the GO-GAs have been successfully confined on the SPE surfaces.
The cathodic to anodic peak current separation for AQ-OH is 50 mV, whereas those for the GAs 1, 2, 3 and 4 are 100 mV, 96 mV, 74 mV and 56 mV, respectively, indicating that the electron transfer (ET) process of the former is kinetically faster than those of the latter. This could be ascribed to the presence of the triazolyl glycosides on the AQ moiety, impeding the ET. We also observed that the reduction peak potential (−0.58 V) of AQ-OH centered more negatively than those of the GAs 1–4 (~−0.43 V), suggesting that the latter can be reduced more easily than the former31. The surface coverage concentration for SPE of GAs 1, 2, 3 and 4 were determined to be 4.4 × 10−9 mol cm−2, 5.0 × 10−9 mol cm−2, 3.5 × 10−9 mol cm−2 and 4.2 × 10−9 mol cm−2, respectively. However, addition of GO-free GA1 (Fig. S4a) and GA2 (Fig. S4b) to SPE led to apparently decreased peak currents, suggesting that GO can more compactly cluster the electroactive GAs on the electrode.
Function of the biomimetic interface
With the biomimetic interfaces in hand, their sensing ability was first tested in a Tris-HCl buffer (pH 7.0) containing Ca2+ and Mn2+ via differential pulse voltammetry (DPV) for lectins, proteins that contain sugar-recognition domains32. Primarily, addition of the mannose/glucose-specific Concanavalin A (Con A, 10 μM) and galactose-specific peanut agglutinin (PNA, 10 μM) to GO-free SPEs doped with GA1 and GA2, respectively, led to a notable current quenching (by ~60%, Fig. S4). However, addition of the lectins reversely also decreased the current intensity (by ~15%), suggesting that both the specific sugar-lectin recognitions and non-specific protein adsorptions on the electrode surface might have contributed to the signal decay.
Subsequently, we interrogated the biospecificity of the SPEs confined with the GO-GAs. Incubation of the specific lectin (8 μM) with the corresponding SPE (Con A to the glucose-confined 1 and 3 and PNA to the galactose-confined 2 and 4) led to a clear current quenching, whereas the addition of the two lectins reversely did not induce noticeable current change (Fig. 3). Moreover, the presence of a panel of other non-specific lectins (8 μM) including the N-acetyl-galactosamine-specific soybean agglutinin (SBA), the fucose-specific Ulex europeaus agglutinin (UEA-I), the N-acetyl-glucosamine-specific wheat germ agglutinin (WGA) and the mannose-specific Pisum satiwun agglutinin (PSA) caused trivial current alternations of the SPEs confined with GO-GAs 1 and 2 (Fig. 3). We note that, however, the addition of SBA to the galactose-confined SPE 2 led to a minor current quenching; because besides N-acetyl-galactosamine, this lectin also binds with its structurally analogous galactose with a lesser affinity. Pre-incubation of free methyl O-mannoside with Con A and methyl O-galactoside with PNA inhibited the current quenching of 1 and 2, respectively, confirming that the signal is derived from specific sugar-lectin interactions (Fig. S5). These data positively corroborate that the SPEs confined with GO-GAs are bio-specific.
The peak current of the SPEs decreased gradually with increasing concentration of a specific lectin (from 1 μM to 25 μM [for details, see Table S1], Fig. 4a–d). Interestingly, the dimeric SPEs 1 (Fig. 4a) and 2 (Fig. 4b) are almost twofold more sensitive than their monomeric counterparts 3 (Fig. 4c) and 4 (Fig. 4d), respectively, which is likely due to the denser sugar clustering at the interface of the former. The limits of detection of 1–4, determined as 16, 25, 88 and 69 nM, respectively (S/σb = 3, where σb is the standard deviation of the peak current obtained in the absence of an analyte), also illustrate that the sensitivity of the dimer-confined is better than the monomer-confined SPEs.
To better describe the sugar-lectin interactions at the interfaces, electrochemical impedance spectroscopy (EIS) which is a reliable method for measuring the resistive ability of an interfacial substance was performed using [Fe(CN)6]3-/4- as a redox probe (Fig. 4)33. The Nyquist plots for bare electrodes exhibited an irregular semicircle at higher frequencies indicating minor electron transfer resistance (Ret) and a straight line at lower frequencies that represents the ET process limited by diffusion. While GO was spotted to the electrode surface, additional semicircles indicative of electrochemical reactions between the electrode and a GO-film were recorded. Deposition of GO-GAs 1 and 2 to the electrodes led to further enlarged capacitive loops due to the formation of compound films at the interface. In particular, incubation of Con A with 1 and PNA with 2 (15 μM) similarly resulted in a much more increased loop, which means that an additional lectin film was probably established at the interface by binding to the densely exposed sugar cluster, causing a greated Ret. Addition of a non-specific lectin to the SPE (i.e. PNA to 1 and Con A to 2) led to, however, insignificant Ret increment. These data imply that the quenched peak current of the SPEs acquired by DPV is plausibly caused by “encapsulation” of the glycosyl quinones by lectin34 leading to impaired ET.
Eventually, the ability of the interfacial system to probe sugar-based ligand-receptor interactions was examined with a human hepatocellular carcinoma cell line (Hep-G2) expressing galactose-selective asialoglycoprotein receptors (ASGP-R)35. Incubation of Hep-G2 cells with the galactose-confined SPE 2 led to gradually quenched peak currents (Fig. 5a), implying that the ET process of the galactosyl quinones might be blocked by cells adsorbed at the interface. To verify whether the current decay was caused by cell adsorption as mediated by the specific galactose-ASPG-R recognition, knockdown of ASGP-R1 of Hep-G2 was carried out by RNA interference.
The knockdown assay was performed by transfecting the cells with an ASGP-R1-specific siRNA oligonucleotide duplex or a scrambled siRNA that does not cause gene silencing as control. Quantitative real-time polymerase chain reaction (RT-PCR) of ASGP-R mRNA indicated that transient transfection of Hep-G2 cells with ASGP-R1-targeting siRNA duplex reduced its transcription level by 70%, whereas a fully retained gene expression was obtained while scrambled siRNA was incubated with Hep-G2 (Fig. 5b)36.
The modified cells were then added to 2 over the same concentration range as employed above. We observed that the presence of increasing control cells caused a gradual current drop with quenching rates approximately identical to those induced by wild-type Hep-G2 (Fig. 5c). Nevertheless, the current quenching of 2 upon addition of the Hep-G2 cells with decreased ASGP-R1 weakened apparently throughout the same cellular concentrations (Fig. 5d). Furthermore, incubation of wild-type Hep-G2 with excessive free methyl O-galactosides prior to addition to the SPE resulted in inhibition of the current quenching (Fig. S9b-e). These data suggest that the receptor-ligand recognition indeed contributes to the specific cell capture of the system. Meanwhile, incubation of the glucose-confined SPE 1 with Hep-G2 cells did not cause any current quenching (Fig. S9a,b), indicating the good biospecificity of the biomimetic interfaces at the cellular level.
Unlike conventional glycoarray methods, the technique presented here does not require heavy workload to construct the sensing platform and is free of analyte-labelling, a step that might compromise the bio-specificity. By taking advantage of the unique feature of the prepared GAs bearing an anthraquinone moiety that serves both as an electroactive reporter (to produce a signal) and an aromatic tail that self-assembles to the surface of GO (to realize sugar clustering that reinforces the binding avidity with proteins), biomimetic interfaces were simply fabricated based on easily made SPEs. These interfacial systems have proven to be suitable for sensitive and selective detection of label-free lectins and, especially, cells expressing a specific glycoprotein receptor employing only an inexpensive and portable electrochemical workstation in connection with a personal laptop.
In summary, we have reported on a new tactic for the generation of biomimetic interfaces densely clustered with sugar ligands capable of sensitively capturing glycoprotein receptors on live cancer cells via electrochemical techniques and with economic facilities. The development of this system simply requires a one-step GO-mediated self-assembly of “electrified” anthraquinonyl glycosides to SPEs. We believe that our system due to its simplicity, effectiveness and, especially, admirable biospecificity will become a promising approach for the elaboration of sugar-based ligand-receptor interactions of biological interest.
Cyclic voltammetry (CV)
CVs were recorded with a computer controlled CHI 1232 electrochemical station (Chenhua Co. Ltd, Shanghai, China) between −0.8 V and 0 V (vs. Ag/AgCl) at scan rates ranging from 0 to 120 mV/s. The electrolyte (PBS, 0.5 M, pH = 7.0) used was degassed with N2 for 20 min before measurements. Screen-printed electrodes (SPEs) were pretreated in PBS (0.05 M, pH 7.0) containing 0.1 M KCl by applying an anodic potential of 2 V (vs. Ag/AgCl) for 2 s and were then washed with water three times. The circular area (2 mm in diameter) was used as the working electrode; the reference electrode was printed with 40% AgCl in silver paste and the auxiliary electrode printed with carbon ink. For functionalization of the SPEs, a drop (3 μL) of the water solution of GO-GAs was dripped onto the working electrode and then dried under vacuum. The functionalized SPEs were finally immersed into PBS solutions for recording the CVs.
Differential pulse voltammetry (DPV)
DPVs were recorded with an amplitude of 0.5 V, a pulse width of 0.05 s, a standing time of 2 s and a scanning range from −0.7 V to 0.2 V in PBS (0.5 M, pH 7.0) or in Tris-HCl (0.5 M, pH 7.0). For detection of analytes, a drop (3 μL) of the analyte solution (dissolved in PBS or Tris-HCl) of different concentrations was dripped onto the GO-GA functionalized working electrode area of SPE and incubated for 30 min. Then the electrodes were rinsed with the buffer solution three times, dried at room temperature and then immersed in degassed buffer for measurement.
Electrochemical impedance spectroscopy (EIS)
EIS was performed with a ZAHNER apparatus in the presence of the [Fe(CN) 6]3−/[Fe(CN) 6]4− (5 mM) redox couple in 0.1 M KCl solution in the frequency range of 10 mHz to 100000 Hz (perturbation signal: 5 mV). All data collected were fitted with the software ZSimpWin. A solution (3 μL) of GO or GO-GAs was first dripped onto the working electrode area of SPE, dried under vacuum and then the Nyquist plots recorded. For detection of the sugar-lectin interactions, a drop (Tris-HCl, 3 μL) of Con A or PNA solution was dripped onto the GO-GA functionalized electrodes, dried under vacuum, followed by recording of the Nyquist plots.
The Hep-G2 cells were obtained from ATCC (Rockville, MD), which were cultured in a Dulbecco's Modified Eagle's Medium (Invitrogen, Carlsbad, CA, USA) supplemented with a 10% Fetal bovine serum (Gibco, Gland Island, NY, USA) at 37°C in a 5% humidified CO2 air environment.
Silencing of ASGP-R1 gene by RNA interference
Silencing of the ASGP-R1 gene expression on Hep-G2 cells was mediated by an ASGPR-1 gene-specific or a scrambled (control) siRNA oligonucleotide duplex purchased from Santa Cruz Biotechnologies (Santa Cruz, CA). Briefly, HepG2 cells were seeded at 400,000 cells/well in a 6-well plate and lipofectamine 2000 (Invitrogen) was used for transfection as instructed by the manufacturer. After incubating the cells with siRNAs for 6 h, the lipofetamine-siRNA-complex containing medium was replaced. After another 48 h, the cells were harvested for experiments and the level of ASGP-R1 mRNA was evaluated by quantitative real-time PCR.
Detection of ASGPR mRNA by quantitative RT-PCR
Total cellular RNAs were extracted from HepG2 cells using TRIzol Reagent (Invitrogen) according to the manufacturer's instructions. mRNA was reversely transcribed using the SuperScript First-Strand Synthesis System (TAKARA). For quantitative PCR reactions, 1:10 dilutions of cDNA products were amplified using SYBR Green PCR Master Mix (TAKARA) and analyzed by using ABI Prism 7300 Fast system (Applied Biosystems). All samples were run in triplicate in each experiment. The specificity of detected signals was confirmed by a dissociation curve consisting of a single peak. Values were normalized on the basis of GAPDH mRNA. The forward (fp) and reverse (rp) primers for the reactions were as follows:
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We thank the 973 Project (2013CB733700), the National Science Fund for Distinguished Young Scholars (21125522, 81125023), the National Natural Science Foundation of China (21176076, 21202045), the National Key Science & Technology Specific Projects (012ZX09301-001-004), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA01040303), the Key Project of Shanghai Science and Technology Commission (13NM1400900) and the Fundamental Research Funds for the Central Universities (WK1013002).
The authors declare no competing financial interests.
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Li, Z., Deng, SS., Zang, Y. et al. Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system. Sci Rep 3, 2293 (2013). https://doi.org/10.1038/srep02293
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