Figure 2 : Sustained ROS production is essential for regeneration to proceed.

From: Sustained production of ROS triggers compensatory proliferation and is required for regeneration to proceed

Figure 2

(a) Inhibition of NADPH oxidase with VAS2870 1 μM or DPI 1 μM reduced the size of the regenerate at 72 hpa. Representative images are shown. The efficiency of regeneration was quantified at 3 dpa (day post amputation). The surface of the blastema was measured and subsequently divided using the square length of the amputation plane for each fish. The efficiency of regeneration is expressed as a percentage of the control. (b) Gene expression was analyzed through quantitative RT-PCR on the regenerated fin at 18 hpa after VAS2870 (1 μM) or vehicle (DMSO) treatment. The DMSO-treated sample was taken as 1. (c, d) Quantification of phospho-c-JUN was performed on western blots. Full-length western blots are presented in Supplementary Figure 5. (d) The inhibition of NADPH oxidases (NOX) with VAS2870 (1 μM) or JNK activity with SP600125 (5 μM) reduced c-Jun phosphorylation at 6 hpa. (e) The inhibition of ROS (VAS2870 1 μM) production decreased apoptosis at 18 hpa. In representative pictures, dotted lines indicate amputation plane. The error bars represent the SEM (* p<0.05, **p< 0.01, ***p < 0.001).