Figure 1

From: Doublesex target genes in the red flour beetle, Tribolium castaneum

Figure 1

(A) Schematic representation of isoforms of Tcdsx pre-mRNA, showing the primer positions and regions used for preparation of dsRNA. Boxes show exons and lines show introns. The sizes (bp) of different exons are shown within the exons. Blue colored regions represent the ORF whereas the orange colored regions represent UTRs. Four different splice variants of Tcdsx pre-mRNA, three female- (Tcdsxf1, Tcdsxf2 and Tcdsxf3) and one male-specific (Tcdsxm), are produced. A(n) show the polyadenylation site. Vertical lines represent start and stop codon sites. Vertical arrows show the domains of the TcDsx proteins whereas horizontal arrows show primer positions. Green horizontal lines show regions corresponding to dsRNA; ds-dsxC (common) = 354 bp, ds-dsxf1+f2 = 78 bp and ds-dsxf2 = 120 bp. Primers F1 and R1 were used to amplify full length Tcdsx transcripts and primer qRTCF was used with either qRTf1R, qRTf2R or qRTf3R in qPCR for the quantification of specific Tcdsxf transcripts. The sequences of all the primers mentioned here are given in supplementary Table 2. (B) Gel picture showing three bands (Tcdsxf1, Tcdsxf2 and Tcdsxf3) in females and one band in male (Tcdsxm) as a result of RT-PCR using sex-specific cDNA as template and primers (F1 and R1) specific to ends of Tcdsx (Fig. 1A). M represents DNA size marker. C) Gel picture showing three bands (Tcdsxf1, Tcdsxf2 and Tcdsxf3) in females and one band in male (Tcdsxm) as a result of RT-PCR using sex-specific cDNA as template and internal primers (F2 and R2) spanning the alternatively spliced region of Tcdsx (Fig. 1A). Same primers (F2 and R2) were used for analyzing the splicing status of Tcdsx in previous paper55. M represents DNA size marker.