Figure 4 : The metabolic process of fatty acids and incorporation of label into the TCA cycle from [1-13C8] octanoate.

From: A medium-chain fatty acid as an alternative energy source in mouse preimplantation development

Figure 4

(A) Schematic diagram of fatty-acid metabolic process. Long-chain fatty acids are catalyzed by ACSL1, 3–6 (acy-CoA synthetase long-chain 1, 3–6) and CPT1 (carnitine O-palmitoyltransferase 1). Then, long-chain fatty acids are transferred from the cytosol to the mitochondria by CACT (carnitine acylcarnitine translocase) for subsequent β-oxidation. On the other hand, medium-chain fatty acids enter directly into the mitochondrial matrix, and subsequently acyl-CoAs are synthesized. Acyl-CoA-fatty acid products are dehydrogenased by ACADM and ACADL, and the products are subjected to the β-oxidation spiral. (B) Western blotting analysis of ACADM, ACADL, and HADHA in oocytes, 8-cell embryos, and blastocysts (U: unfertilized oocyte, 8: 8-cell embryo, and B: blastocyst). Actin was used as a loading control. An amount of extracted protein corresponding to 100 oocytes or embryos was loaded per lane. The representative result is shown from three independent experiments. (C) Incorporation of 13C label into TCA-cycle metabolites in mitochondria from [1-13C8] octanoate-containing culture media. Box plot showing the amount of metabolites derived from octanoate (shaded boxes) and [1-13C8] octanoate (open boxes) cultures. Error bars represent SD. N.D. means that the metabolite concentration was below the detection limit of the analysis. P-value was calculated by Student's t-test (*P < 0.05 and ** P < 0.01).