Figure 3 : Effect of octanoate on preimplantation development in fatty acid-deficient culture media or in media lacking glucose, pyruvate, and fatty acids.

From: A medium-chain fatty acid as an alternative energy source in mouse preimplantation development

Figure 3

The beneficial effect of octanoate supplementation on preimplantation development was clearly observed in both experimental conditions, using fatty acid-deficient and energy-depleted media. Embryos were cultured in (a) fatty acid-containing KSOM, (c) fatty acid-deficient media or (b) fatty acid-deficient and 100 µM octanoate-supplemented media (A–C). Alternatively, embryos were cultured in (e) media lacking glucose (Glu), pyruvate, and fatty acids (energy-depleted media) or (d) energy-depleted and 100 µM octanoate-supplemented media (D–F). A vertical scale in each graph shows the developmental rate from 1-cell embryos to 8-cell embryos/morulae at 2.5 d.p.c. (A, C), from 8-cell embryos to blastocysts at 4.5 d.p.c. (B, E), or from hatched blastocysts at 5.5 d.p.c. (C, F). Results are shown as the mean % ± SEM, n > 4 experimental replicates, unpaired t-test performed within developmental stage; *P < 0.05 and ** P < 0.001). N.S. means not significant.