Normal cell cycle progression in asynchronous Cdc14b-null cells.
(a) Primary MEFs were transduced with lentiviruses expressing Histone H2B-GFP and cells were recorded by videomicroscopy. Time 0 indicates mitotic entry. No differences are found in the duration of mitosis in wild-type or Cdc14b-null cells. (b) Growth curve of Cdc14b-null and control primary MEFs during 6 days. (c) Percentage of mitotic cells in these asynchronous cultures. (d) Mitotic exit kinetics in Cdc14b-null (N = 84) and control (N = 67) cells in the presence of nocodazole. Cells were arrested in prometaphase with nocodazole and the percentage of mitotic cells, as observed by videomicroscopy, is scored showing no differences between these two genotypes during mitotic slippage. All these graphs show representative results from at least three separate experiments. (e) Immunofluorescence staining of passage 7 (P7) primary MEFs and quantification of the percentage of γH2AX positive cells in primary MEFs at passages 3, 5 and 7. (f) Immunofluorescence staining of passage 3 (P3) primary MEFs after 10 Gy of γ−irradiation. Quantification of γH2AX-positive cells and the average γH2AX intensity per cell shows no significant differences between wild-type and Cdc14b-null cultures. All these graphs show results from three separate experiments.