Figure 4

From: Inhibition of Mcl-1 enhances cell death induced by the Bcl-2-selective inhibitor ABT-199 in acute myeloid leukemia cells

Figure 4

A-1210477 induces DNA damage in acute myeloid leukemia (AML) cells. (a) THP-1, U937, and a primary AML patient sample cells (AML#110) were treated with ABT-199 and A-1210477, alone or in combination, for 4 h or 24 h. Whole-cell lysates were subjected to Western blotting and probed with the indicated antibody. Relative densitometry measurements of γH2AX expression were measured using Odyssey Software V3.0. (b) U937 cells were treated with ABT-199 and A-1210477, alone or in combination, for 4 h and then subjected to alkaline comet analyses. Representative images are shown. (c) Alkaline comet assay results are graphed as median percent DNA in the tail from four replicate gels±s.e.m. *P<0.05 and **P<0.01.