Table 3 Possible states for cell annotation.

From: Phase contrast time-lapse microscopy datasets with automated and manual cell tracking annotations

Status Number Status Name Status Description Recommended Usage
0 Invalid Invalid cell status This state may be applied to indicate error.
1 New The cell appears for the first time in the image sequence. This state may be applied to cells with normal status in the very first frame of the image sequence.
2 Newborn The cell is a newborn (resulting from cell division). This state may be applied to the first frame when newborn daughter cells have appeared as a result of cell division.
3 Divided The cell divided. This state may be applied to the last frame when mitotic cells have divided.
4 Normal The cell does not have any particular event associated with it. This state may be applied for the frame duration when the status of the cell cannot be determined by any other status code.
5 Apoptotic/Mitotic The cell is either apoptotic or mitotic. This state may be applied for the frame duration when its exact status with regards to apoptosis and mitosis cannot be determined (due to similarities in the morphological appearance of cells during apoptosis and mitosis).
6 Apoptotic The cell is apoptotic. This state may be applied for the frame duration when the cell is undergoing apoptosis.
7 Mitotic The cell is mitotic. This state may be applied for the frame duration when the cell is undergoing mitosis.
8 Maybe Dead The cell maybe dead. This state may be applied for the frame duration when its exact status with regards to cell death cannot be determined. This state reflects uncertainty in cell trajectories.
9 Dead The cell is dead. This state may be applied to the last frame of a cell undergoing apoptosis and/or for the frame duration the dead cell debris persists in the image sequence.
10 Fused The cells adjacent to each/one another have fused and are now indistinguishable. This state may be applied to the last frame of a cell prior to fusing as well as for the remaining frame duration of its fusing partner cell. The fusing partner cell’s trajectory will continue being tracked until its cell status changes (e.g. the cell undergoes mitosis, departs the field-of-view, etc).
11 Departed The cell left the field-of-view. This state may be applied to the last frame of a cell prior to departing the field-of-view.
12 Entered The cell entered the field-of-view. This state may be applied to the first frame of a cell as it enters the field-of-view.
13 Appeared A cell has appeared. This state may be applied to the first frame when a new cell whose appearance cannot be explained by mitosis or a cell entering the field-of-view. This state reflects uncertainty in cell trajectories.
14 New Group The cell joins or has formed a group. This state may be applied the first frame when two or more cells can no longer be tracked as single cells and are instead tracked as a group. This state reflects difficulty in assigning cell trajectories.
15 Grouped The cell is in a group. This state may be applied for the remaining frame duration when two or more cells can no longer be tracked as single cells and are instead tracked as a group. This state reflects difficulty in assigning cell trajectories.
16 Differentiated From the Group The cell has left the group. This state may be applied to the first frame when a cell has left a group of cells. This state reflects difficulty in assigning cell trajectories.
17 Maybe Lost The cell maybe lost. This state may be applied for the frame duration when a cell’s trajectory is tentative and uncertain. This state reflects uncertainty in cell trajectories.
18 Lost The cell has been lost. This state may be applied to the last frame when a cell’s trajectory can still be tracked. This state reflects difficulty in assigning cell trajectories.