The minimum, maximum, modal, and mean pixel values (arbitrary units) for each individual image of the sequence was plotted as a function of image frame number. The mean, minimum, and maximum pixel intensity remained relatively unchanged throughout the image sequence. Addition of FGF2 resulted in cell morphology changes that increased pixel intensity. Each dataset consists of four culture conditions – Control (growth media), 50 ng/mL FGF2, 100 ng/mL BMP2, and 50 ng/mL FGF2 + 100 ng/mL BMP2. Four replicates were imaged per dataset at a frequency of 5 minute intervals over approximately 87.0 h.