Table 3 Channel specification Description of which cell population is expected to be visible in each channel of the provided videos.

From: Leukocyte Tracking Database, a collection of immune cell tracks from intravital 2-photon microscopy videos

VideoID CH0 CH1 CH2 CH3
CS001 BG(FR) Tc(CMTMR) a:Tc(HIV-GFP) Coll
CS002 BG(FR) Tc(CMTMR) a:Tc(HIV-GFP) Coll
CS003 BG(FR) Tc(CMTMR) a:Tc(HIV-GFP) Coll
CS004 a:Ne(UBC-CFP), Dc(CD11c YFP) Vaccine a:Ne(UBC-CFP), Coll  
CS005 a:Ne(UBC-GFP)    
CS006 BG(FR) Tc(CMTMR) a:Tc(HIV-GFP) Coll
CS007 a:Ne(UBC-GFP)    
CS008 a:Ne(UBC-GFP)    
CS009 a:Ne(UBC-GFP)    
CS010 a:Ne(UBC-CFP), Dc(CD11c YFP) Vaccine a:Ne(UBC-CFP), Coll  
CS011 a:Ne(UBC-GFP), BG BG Coll, BG  
CS012 a:Tc(CFSE)    
CS013 a:Ne(UBC-CFP), Dc(CD11c YFP) Vaccine a:Ne(UBC-CFP), Coll  
CS014 a:Ne(UBC-GFP) Vaccine a:Ne(UBC-CFP), Coll  
CS015 a:Ne(UBC-CFP), Dc(CD11c YFP), AF Ne(CMTMR), AF a:Ne(UBC-CFP), Coll, AF  
CS016 a:Ne(UBC-CFP), Dc(CD11c YFP), AF Ne(CMTMR), AF a:Ne(UBC-CFP), Coll, AF  
CS017 a:Ne(UBC-CFP), Dc(CD11c YFP), AF Ne(CMTMR), AF a:Ne(UBC-CFP), Coll, AF  
CS018 a:NK(NCR1 GFP), Mp(CD169 Pe) Mp(Cd169 Pe) Vaccine Coll
LTDB001 a:Ne(UBC-CFP), Dc(CD11c YFP) Vaccine a:Ne(UBC-CFP), Coll  
LTDB002 a:Ne(UBC-CFP), Dc(CD11c YFP) Vaccine a:Ne(UBC-CFP), Coll  
LTDB003 a:Ne(UBC-CFP), Dc(CD11c YFP) Vaccine a:Ne(UBC-CFP), Coll  
LTDB004 Ne(UBC-GFP), a:Ne(UBC-CFP), AF b:Ne(CMTMR), AF a:Ne(UBC-CFP), Coll, AF  
LTDB005 Ne(UBC-GFP), a:Ne(UBC-CFP), AF b:Ne(CMTMR), AF a:Ne(UBC-CFP), Coll, AF  
LTDB006 a:Ne(UBC-CFP), Dc(CD11c YFP) Vaccine, BG a:Ne(UBC-CFP), Coll  
LTDB007 b:Ne(UBC-GFP), a:Ne(UBC-CFP), AF Ne(CMTMR), AF a:Ne(UBC-CFP), Coll, AF  
LTDB008 a:Ne(UBC-CFP), Dc(CD11c YFP) Vaccine a:Ne(UBC-CFP), Coll  
LTDB009 a:Ne(UBC-CFP), Dc(CD11c YFP) Vaccine a:Ne(UBC-CFP), Coll  
LTDB010 Ne(UBC-GFP), BG a:Bc(CTV)   
LTDB011 a:Ne(UBC-GFP), BG Bc(CD19 RFP)   
LTDB012 BG(FR) a:Tc(CMTMR) b:Tc(HIV-GFP) Coll
LTDB013 BG(FR) Tc(CMTMR) a:Tc(HIV-GFP) Coll
LTDB014 b:Bc(Bodipy 650) Tc(H2B-RFP) Tc(NFAT-GFP) a:Bc(Ag+ Hoechst33342)
LTDB015 Tc(CMAC) a:Tc(CFSE) Tc(CMTMR) HEV(Meca-633)
LTDB016 a:Ne(UBC-CFP), Dc(CD11c YFP) Ne(CMTMR), AF a:Ne(UBC-CFP), Coll, AF  
LTDB017 a:Ne(UBC-CFP), Dc(CD11c YFP) Ne(CMTMR), AF a:Ne(UBC-CFP), Coll, AF  
LTDB018 Dc(CD11c YFP) a:NK(CMTMR)   
LTDB019 a:NK(CTV), Dc(CD11c YFP) Vaccine a:NK(CTV)  
LTDB020 BG a:NK(CMTMR) BG Coll
  1. Bold indicates the cells that have been tracked. Brackets reports the type of staining used. The prefix a: or b: is used to indicate which is the corresponding tracking file (e.g. for video 04, b:N.(CMTMR) means that the tracks relative to the neutrophils labelled with CMTMR are in 04b.csv). Legend: Bc = B cells, Tc = T cells, Ne = neutrophils, Dc = dendritic cells, NKs= natural killer cells, Mp = macrophages, AF = auto-fluorescence, BG = background, Coll = collagen.