Figure 1 : Samples and workflow.

From: Mass spectrometry quantitation of proteins from small pools of developing auditory and vestibular cells

Figure 1

a, Utricles and cochleas with hair cells marked with GFP were subject to cell dissociation and FACS at three different developmental ages. GFP-positive or GFP-negative cells were separately collected and used for further analysis. b, Pools of 5000 isolated cells collected under the appropriate conditions were denatured, and their proteins were digested to peptides using the protease trypsin and the eFASP preparation method. Identical aliquots were run on a Q Exactive HF mass spectrometry either using data-dependent (DDA) or data-independent (DIA) acquisition.