Figure 3: siRNA depletion is reproducible between replicate plates and over the screen. | Scientific Data

Figure 3: siRNA depletion is reproducible between replicate plates and over the screen.

From: RNAi screens for Rho GTPase regulators of cell shape and YAP/TAZ localisation in triple negative breast cancer

Figure 3

(a) Correlation matrix between Z-scores of well-averaged feature vectors between duplicate plates. The positive distribution (+) represents the correlation between replicate wells and the negative distribution (−) determines the null-value correlation. Pairwise t-tests for the correlation increases from null gave a P value less than 0.001. (b) Pearson correlations between technical replicates and randomly drawn siRNAs. The positive distribution has a median correlation of 0.594, and a strong negative skew of −1.02. The null distribution has a near zero median of 0.169 and no skew (0.02). P<0.001 (pairwise t-test) between the positive distribution and the null distribution. (c) Representative images of the effect of knocking down LATS1 on YAP/TAZ nuclear translocation in LM2 and MDA-MB-231 cells compared to control cells (containing only transfection reagent). The intensity of YAP/TAZ stain in the nucleus is higher than for control cells (white arrows). (d) Representative images of YAP/TAZ translocation to the nucleus as a result of ARHGEF7 knockdown in both the siGENOME and ONTARGETPlus screens in LM2 cells, compared to control cells and siLATS1 transfected cells are included as a comparison. White arrows point to cells whose YAP/TAZ intensity is representative of the condition stated. (e) Representative images of multinuclear cells (white arrows) as a result of ECT2 and RACGAP1 knockdown for LM2 and MDA-MB-231 in both the siGENOME and ONTARGETPlus screens.

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