Figure 1: Experimental workflow. | Scientific Data

Figure 1: Experimental workflow.

From: RNAi screens for Rho GTPase regulators of cell shape and YAP/TAZ localisation in triple negative breast cancer

Figure 1

This study consisted of RNAi screens to isolate RhoGEFs and RhoGAPs that are essential for the morphogenesis of the highly metastatic TNBC cell line LM2, and its less-metastatic parental cell line MDA-MB-231. The screening approach is depicted in a sequential manner starting with the reverse transfection, followed by staining and imaging, and ending on image analysis.

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