Single-cell technology has become an indispensable tool in cardiovascular research since its first introduction in 2009. Here, we highlight the recent remarkable progress in using single-cell technology to study transcriptomic and epigenetic heterogeneity in cardiac disease and development. We then introduce the key concepts in single-cell multi-omics modalities that apply to cardiovascular research. Lastly, we discuss some of the trending concepts in single-cell technology that are expected to propel cardiovascular research to the next phase of single-cell research.
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We acknowledge funding support from American Heart Association grant 20POST35210896 (to W.L.W.T.); F30 HL156478 (to A.Z.); National Institutes of Health (NIH) grants R01 HG010359 (to W.H.W.), and R01 HL145676, R01 HL146690, R01 HL130020, R01 HL145676, R01 HL141371 and R01HL126527 (to J.C.W.); and Chan Zuckerberg Initiative 2020-217708 and 2021-240451 (to J.C.W.). We thank B. C. Wu (Stanford University), D. T. Paik (Stanford University), S. S. Hye (Stanford University) and L. C. J. Mick (National University of Singapore) for critical reading of the manuscript.
W.J.G. is an equity holder of 10x Genomics and a cofounder of Protillion Biosciences and J.C.W. is a cofounder and scientific advisory board member for Greenstone Biosciences, but the work presented here is completely independent. The remaining authors declare no competing interests.
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Tan, W.L.W., Seow, W.Q., Zhang, A. et al. Current and future perspectives of single-cell multi-omics technologies in cardiovascular research. Nat Cardiovasc Res 2, 20–34 (2023). https://doi.org/10.1038/s44161-022-00205-7